Cobbett C S, Delbridge M L
J Bacteriol. 1987 Jun;169(6):2500-6. doi: 10.1128/jb.169.6.2500-2506.1987.
The regulatory region of the aroF-tyrA operon was fused to the chloramphenicol acetyltransferase (cat) gene on a plasmid vector. Expression of the cat gene was subject to repression by tyrR+. This fusion was used to isolate regulatory mutants with increased expression of the cat gene in which repression by tyrR+ was affected. Nucleotide sequencing of these mutants has led to the identification of three sites involved in the repression of aroF by tyrR+. The existence of a functional promoter divergently transcribing from the aroF regulatory region was also demonstrated by using the cat fusion vector. The expression of this promoter is also regulated by tyrR+.
将aroF-tyrA操纵子的调控区域与质粒载体上的氯霉素乙酰转移酶（cat）基因融合。cat基因的表达受到tyrR⁺的抑制。这种融合用于分离cat基因表达增加的调控突变体，其中tyrR⁺的抑制作用受到影响。对这些突变体进行核苷酸测序已鉴定出三个参与tyrR⁺对aroF抑制作用的位点。通过使用cat融合载体也证明了存在一个从aroF调控区域反向转录的功能性启动子。该启动子的表达也受tyrR⁺调控。
