Hamparian V V, Ottolenghi A C, Hughes J H
Appl Environ Microbiol. 1985 Aug;50(2):280-6. doi: 10.1128/aem.50.2.280-286.1985.
We describe our experience with the isolation of viruses from four treatment plants located in different geographic areas. Over a period of 3 years, 297 enteroviruses were isolated from 307 sludge samples. The highest frequency of viral isolation (92%), including multiple isolates from single samples, was obtained from a treatment plant serving the smallest population. Excluding the polioviruses, 22 different enterovirus serotypes were isolated. The methods used to isolate the viruses were relatively simple and included an elution procedure in which beef extract was used and a disinfection step. No concentration procedure was used. Of three cell culture systems used, the RD line of human rhabdomyosarcoma cells was by far the most useful for the isolation of echoviruses; BGM and HeLa cells were particularly useful for the isolation of group B coxsackieviruses. A seasonal effect on viral isolation rates from sludge was observed.
我们描述了从位于不同地理区域的四个污水处理厂分离病毒的经验。在3年的时间里，从307份污泥样本中分离出297株肠道病毒。病毒分离的最高频率（92%），包括从单个样本中分离出多个毒株，来自服务人口最少的污水处理厂。排除脊髓灰质炎病毒后，分离出22种不同的肠道病毒血清型。用于分离病毒的方法相对简单，包括使用牛肉提取物的洗脱程序和消毒步骤。未使用浓缩程序。在所使用的三种细胞培养系统中，人横纹肌肉瘤细胞的RD系对于分离埃可病毒最为有用；BGM细胞和HeLa细胞对于分离B组柯萨奇病毒特别有用。观察到了污泥中病毒分离率的季节性影响。
