两种用于检测鼠冠状病毒抗体的酶免疫测定法。
Two enzyme immunoassays for the detection of antibody to rodent coronaviruses.
作者信息
Smith A L, Winograd D F
出版信息
J Virol Methods. 1986 Nov;14(3-4):335-43. doi: 10.1016/0166-0934(86)90035-2.
Abstract
Two enzyme immunoassays for detection of antibody to rodent coronaviruses were compared. Mouse hepatitis virus (MHV), strain JHM, antigen was in the form of formalin-fixed, infected 17 C1 1 cells. This antigen detected antibody to the homologous strain of MHV as well as to two heterologous MHV strains and a serologically related rat coronavirus, sialodacryodenititis virus. Antibody titers in assays using horseradish peroxidase (HRP)-conjugated or ureiase-conjugated anti-mouse IgG were substantially higher than in an indirect immunofluorescence assay. The ureiase assay was somewhat more sensitive than the HRP assay. MHV-JHM antigen was stable under a variety of storage conditions for at least two months.
摘要
比较了两种用于检测鼠冠状病毒抗体的酶免疫测定法。小鼠肝炎病毒（MHV）JHM株抗原为福尔马林固定的、感染的17C11细胞形式。该抗原可检测到针对MHV同源株以及两种异源MHV株和一种血清学相关的大鼠冠状病毒——涎泪腺炎病毒的抗体。使用辣根过氧化物酶（HRP）偶联或脲酶偶联的抗小鼠IgG进行测定时的抗体滴度显著高于间接免疫荧光测定法。脲酶测定法比HRP测定法稍敏感。MHV-JHM抗原在多种储存条件下至少两个月内稳定。
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