通过酶联免疫吸附测定法检测4种鼠冠状病毒抗原与免疫或自然感染大鼠血清的反应性。

Reactivities of 4 murine coronavirus antigens with immunized or naturally infected rat sera by enzyme linked immunosorbent assay.

作者信息

Machii K, Iwai H, Otsuka Y, Ueda K, Hirano N

机构信息

Department of Veterinary Public Health, Institute of Public Health, Tokyo, Japan.

出版信息

Jikken Dobutsu. 1988 Jul;37(3):251-5. doi: 10.1538/expanim1978.37.3_251.

DOI:10.1538/expanim1978.37.3_251

PMID:2843391

Abstract

Four murine coronavirus antigens, sialodacryoadenitis virus (SDAV) strain TG, Parker's rat coronavirus (PCV) strain 8190, mouse hepatitis virus (MHV) strains S and NuU, were examined for their reactivities to hyperimmunized and naturally infected rat sera by ELISA. With the immunized sera, SDAV and PCV antigens reacted best with respective homologous sera. MHV antigens reacted with all antisera, anti-SDAV, anti-PCV, and anti-MHV-S at approximately the same level, and MHV-S showed a slightly higher reactivity than MHV-NuU. The reactivities of the sera from various colonies to these antigens were in the order--from high to low--of SDAV, MHV-S, MHV-NuU, and PCV. None of sera negative for SDAV antigen reacted positively to the other antigens. Within the sera positive for SDAV, the positivities were in the order of MHV-S, MHV-NuU, and PCV. These results suggested that, although homologous antigens are best to detect SDAV or PCV infection by ELISA, MHV antigen can be used if highly cross-reactive viral strain is selected.

摘要

通过酶联免疫吸附测定（ELISA）检测了四种鼠冠状病毒抗原，即涎泪腺炎病毒（SDAV）TG株、帕克大鼠冠状病毒（PCV）8190株、小鼠肝炎病毒（MHV）S株和NuU株，与经超免疫和自然感染的大鼠血清的反应性。对于免疫血清，SDAV和PCV抗原与各自的同源血清反应最佳。MHV抗原与所有抗血清，即抗SDAV、抗PCV和抗MHV-S的反应水平大致相同，且MHV-S的反应性略高于MHV-NuU。来自不同菌落的血清对这些抗原的反应性顺序为——从高到低——SDAV、MHV-S、MHV-NuU和PCV。对SDAV抗原呈阴性的血清对其他抗原均无阳性反应。在对SDAV呈阳性的血清中，阳性顺序为MHV-S、MHV-NuU和PCV。这些结果表明，虽然通过ELISA检测SDAV或PCV感染时同源抗原最佳，但如果选择高度交叉反应的病毒株，MHV抗原也可使用。