Ligeon Laure-Anne, Loi Monica, Münz Christian
Department of Viral Immunobiology, Institute of Experimental Immunology, University of Zurich, Zurich, Switzerland.
Curr Protoc Immunol. 2018 Nov;123(1):e60. doi: 10.1002/cpim.60. Epub 2018 Sep 25.
LC3-associated phagocytosis (LAP) is an unconventional form of autophagy that relies on parts of the canonical autophagy machinery for its function. LAP is triggered upon receptor-mediated phagocytosis and is characterized by the formation of a single-membrane vesicle decorated with the autophagy protein LC3. In professional phagocytic cells, such as macrophages, the role of LAP in immune processes has been characterized, although how LAP functions at the molecular level remains poorly defined. It is important to point out that as for all autophagic pathways, the study of LAP is still challenging for the scientific community because it is a dynamic and complex process, requiring interactions among several proteins. Here, we describe the most common methods used to monitor and quantify the formation of LC3-coated single-membrane endosomes, or so-called LAPosomes, and to validate the involvement of LAP in immunological processes of human macrophages. © 2018 by John Wiley & Sons, Inc.
LC3相关吞噬作用（LAP）是一种非传统的自噬形式，其功能依赖于经典自噬机制的部分成分。LAP在受体介导的吞噬作用时被触发，其特征是形成一个由自噬蛋白LC3修饰的单膜囊泡。在专业吞噬细胞（如巨噬细胞）中，LAP在免疫过程中的作用已得到明确，但LAP在分子水平上的功能仍不清楚。需要指出的是，对于所有自噬途径而言，LAP的研究对科学界来说仍然具有挑战性，因为它是一个动态且复杂的过程，需要多种蛋白质之间的相互作用。在此，我们描述了用于监测和定量LC3包被的单膜内体（即所谓的LAP小体）形成，并验证LAP参与人类巨噬细胞免疫过程的最常用方法。© 2018约翰威立国际出版公司
