Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos 62210, Mexico.
Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos 62210, Mexico.
Virology. 2018 Dec;525:96-105. doi: 10.1016/j.virol.2018.09.013. Epub 2018 Sep 22.
Cellular mRNAs cycle between translating and non-translating pools, polysomes compose the translating pool, while RNA granules contain translationally-silenced mRNAs, where the RNAs are either stored in stress granules, or accumulate in processing bodies (PBs) or GW-bodies, which have an important role in RNA degradation. Viruses have developed measures to prevent the deleterious effects of these structures during their replication. Rotavirus, the most common agent of viral gastroenteritis, is capable of establishing a successful infection by counteracting several of the antiviral responses of its host. Here, we describe that in rotavirus-infected cells the distribution of several RNA binding proteins is changed causing the disaggregation of PBs, the relocalization of GW-body proteins, and the cytoplasmic accumulation of HuR, a predominantly nuclear protein. We show that this redistribution of proteins is more likely caused by the accumulation of viral RNA in the cytoplasm of infected-cells, where it might be acting as an RBP sponge.
细胞 mRNA 在翻译和非翻译池之间循环，多核糖体组成翻译池，而 RNA 颗粒包含翻译沉默的 mRNA，其中 RNA 要么储存在应激颗粒中，要么在处理体 (PBs) 或 GW 体中积累，这些在 RNA 降解中起重要作用。病毒已经开发出措施来防止这些结构在复制过程中产生的有害影响。轮状病毒是病毒性胃肠炎最常见的病原体，它能够通过对抗宿主的几种抗病毒反应来建立成功的感染。在这里，我们描述了在轮状病毒感染的细胞中，几种 RNA 结合蛋白的分布发生改变，导致 PBs 解聚、GW 体蛋白重新定位以及 HuR（一种主要存在于核内的蛋白）的细胞质积累。我们表明，这种蛋白质的重新分布更可能是由感染细胞中病毒 RNA 的积累引起的，在感染细胞中，病毒 RNA 可能作为 RBPs 海绵发挥作用。
