Mosser D M, Vlassara H, Edelson P J, Cerami A
J Exp Med. 1987 Jan 1;165(1):140-5. doi: 10.1084/jem.165.1.140.
In this paper we demonstrate the involvement of the macrophage receptor for advanced glycosylation endproducts (AGE) in the phagocytosis of Leishmania major promastigotes. Blocking of this receptor with the ligand, AGE-BSA, leads to a 50% decrease in phagocytosis relative to controls, and a comparable decrease in the respiratory burst. The inhibition of phagocytosis by AGE-BSA was specific to leishmania. The binding of zymosan or C3bi-RBC and the phagocytosis of IgG-RBC or latex beads was not affected by the presence of AGE-BSA. Blocking of both the AGE receptor and CR3 decreases leishmania binding by nearly 90%, and reduces the respiratory burst by 80%, indicating that the two receptors account for the bulk of L. tropica promastigote recognition and uptake by the macrophage.
在本文中，我们证明了晚期糖基化终产物（AGE）巨噬细胞受体参与了巨噬细胞对利什曼原虫前鞭毛体的吞噬作用。用配体AGE-BSA阻断该受体后，相对于对照组，吞噬作用降低了50%，呼吸爆发也有类似程度的降低。AGE-BSA对吞噬作用的抑制作用对利什曼原虫具有特异性。酵母聚糖或C3bi-RBC的结合以及IgG-RBC或乳胶珠的吞噬作用不受AGE-BSA的影响。同时阻断AGE受体和CR3可使利什曼原虫的结合减少近90%，并使呼吸爆发降低80%，这表明这两种受体在巨噬细胞对热带利什曼原虫前鞭毛体的识别和摄取中起主要作用。
