Faculty of Chemistry, Bioanalysis Laboratory, University of Bialystok, Ciolkowskiego 1K, 15-245 Bialystok, Poland.
Faculty of Chemical Technology, Poznan University of Technology, Pl. Sklodowskiej-Curie 5, 60-965 Poznan, Poland.
Biosensors (Basel). 2023 Feb 16;13(2):279. doi: 10.3390/bios13020279.
Diagnostics based on the determination of biomarkers in body fluids will be more successful when several biomarkers are determined. A multiple-array SPRi biosensor for the simultaneous determination of CA125, HE4, CEA, IL-6 and aromatase has been developed. Five individual biosensors were placed on the same chip. Each of them consisted of a suitable antibody covalently immobilized onto a gold chip surface via a cysteamine linker by means of the NHS/EDC protocol. The biosensor for IL-6 works in the pg mL range, that for CA125 in the µg mL range, and the other three within the ng mL range; these are ranges suitable for the determination of biomarkers in real samples. The results obtained with the multiple-array biosensor are very similar to those obtained with a single biosensor. The applicability of the multiple biosensor was demonstrated using several examples of plasma from patients suffering from ovarian cancer and endometrial cyst. The average precision was 3.4% for the determination of CA125, 3.5% for HE4, 5.0% for CEA and IL-6, and 7.6% for aromatase. The simultaneous determination of several biomarkers may be an excellent tool for the screening of the population for earlier detection of diseases.
基于体液中生物标志物的诊断在确定多个生物标志物时会更成功。已经开发出一种用于同时测定 CA125、HE4、CEA、IL-6 和芳香酶的多阵列 SPRi 生物传感器。五个单独的生物传感器被放置在同一个芯片上。它们中的每一个都由通过 NHS/EDC 方案通过半胱氨酸连接子共价固定在金芯片表面上的合适抗体组成。用于 IL-6 的生物传感器在 pg mL 范围内工作，用于 CA125 的在 µg mL 范围内，其他三个在 ng mL 范围内；这些是适合在实际样品中测定生物标志物的范围。多阵列生物传感器获得的结果与单个生物传感器获得的结果非常相似。使用来自患有卵巢癌和子宫内膜囊肿的患者的几种血浆的实例证明了多生物传感器的适用性。CA125 测定的平均精密度为 3.4%，HE4 为 3.5%，CEA 和 IL-6 为 5.0%，芳香酶为 7.6%。同时测定多个生物标志物可能是用于人群筛查以更早发现疾病的极好工具。
