Fujino N, Kimura M, Sakamoto K, Shigaki N, Yamashita J, Akagi M
Jpn J Surg. 1986 Sep;16(5):311-7. doi: 10.1007/BF02470552.
Tamoxifen binding sites (TBS) were measured using 3H-tamoxifen, the objective being to evaluate the relationships among TBS and hormone receptors and/or clinical and pathological characteristics in malignant tissues from 60 patients with mammary cancer. TBS were detected in most (96.7 per cent) cancers in the breast tissues, and the mean content and affinity were 569 fmol/mg X protein with Kd: 1.98 nM. There was no significant correlation between TBS and the estrogen receptor and/or progesterone receptor, with respect to positivity or content. However, there was a significant correlation between TBS and histological grading, thereby indicating the differentiation and the proliferative activity in this tissue. The content of TBS was significantly higher in the group with a high grade of malignancy. The TBS content significantly increased in parallel with the degree of malignancy, as related to tubule formation, nuclear pleomorphism and mitotic activity. On the other hand, there was no significant correlation between TBS and age, tumor size, lymph node status or clinical stage. These results suggest the possibility that TBS may be associated with differentiation and cell-proliferation in breast cancer tissues.
使用³H-他莫昔芬测定他莫昔芬结合位点（TBS），目的是评估60例乳腺癌患者恶性组织中TBS与激素受体和/或临床及病理特征之间的关系。在大多数（96.7%）乳腺组织癌中检测到TBS，平均含量和亲和力分别为569 fmol/mg X蛋白，解离常数（Kd）为1.98 nM。就阳性或含量而言，TBS与雌激素受体和/或孕激素受体之间无显著相关性。然而，TBS与组织学分级之间存在显著相关性，从而表明该组织的分化和增殖活性。恶性程度高的组中TBS含量显著更高。与小管形成、核多形性和有丝分裂活性相关，TBS含量随恶性程度升高而显著增加。另一方面，TBS与年龄、肿瘤大小、淋巴结状态或临床分期之间无显著相关性。这些结果提示TBS可能与乳腺癌组织中的分化和细胞增殖有关。
