Yoo Jae Young, You Young-Ah, Kwon Eun Jin, Park Mi Hye, Shim Sunah, Kim Young Ju
Department of Obstetrics and Gynecology, Ewha Womans University College of Medicine, Seoul, Korea.
Ewha Medical Institute, Ewha Medical Center, Ewha Womans University College of Medicine, Seoul, Korea.
Obstet Gynecol Sci. 2018 Sep;61(5):565-574. doi: 10.5468/ogs.2018.61.5.565. Epub 2018 Sep 3.
This study aimed to investigate the association between preterm birth and epigenetic mechanisms in the amnion.
We examined the association between differentially methylated regions (DMRs) and differentially expressed genes (DEG) using a cytosine-phosphate-guanine methylation array and whole-transcriptome sequencing from the amnion (preterm birth, n=5; full term, n=5). We enrolled 35 participants for mRNA expression analysis and pyrosequencing: 16 full-term and 19 preterm subjects. We compared the association of integrin subunit alpha 11 () and thrombospondin 2 () gene methylation status with mRNA expression in the amnion.
In the preterm birth group, methylation of and genes was significantly lower ( gene: 60.30% vs. 73.16%, <0.05; gene: 64.59% vs. 73.16%, <0.05), and the expression of the genes was significantly higher than that in the full-term group ( gene: 14.20 vs. 1.57, <0.01; gene: 1.18 vs. 10.34, <0.05).
Methylation of the and genes in the amnion was associated with preterm birth. Thus, and gene methylation status in the amnion may be valuable in explaining the mechanism underlying preterm birth.
本研究旨在探讨早产与羊膜中表观遗传机制之间的关联。
我们使用胞嘧啶 - 磷酸 - 鸟嘌呤甲基化阵列和羊膜全转录组测序（早产组，n = 5；足月组，n = 5）检测差异甲基化区域（DMRs）和差异表达基因（DEG）之间的关联。我们招募了35名参与者进行mRNA表达分析和焦磷酸测序：16名足月受试者和19名早产受试者。我们比较了羊膜中整合素亚基α11（）和血小板反应蛋白2（）基因甲基化状态与mRNA表达之间的关联。
在早产组中，和基因的甲基化显著降低（基因：60.30%对73.16%，<0.05；基因：64.59%对73.16%，<0.05），且基因表达显著高于足月组（基因：14.20对1.57，<0.01；基因：1.18对10.34，<0.05）。
羊膜中和基因的甲基化与早产有关。因此，羊膜中和基因的甲基化状态可能有助于解释早产的潜在机制。
