Differential expression and methylation of integrin subunit alpha 11 and thrombospondin in the amnion of preterm birth.

OBJECTIVE

This study aimed to investigate the association between preterm birth and epigenetic mechanisms in the amnion.

METHODS

We examined the association between differentially methylated regions (DMRs) and differentially expressed genes (DEG) using a cytosine-phosphate-guanine methylation array and whole-transcriptome sequencing from the amnion (preterm birth, n=5; full term, n=5). We enrolled 35 participants for mRNA expression analysis and pyrosequencing: 16 full-term and 19 preterm subjects. We compared the association of integrin subunit alpha 11 () and thrombospondin 2 () gene methylation status with mRNA expression in the amnion.

RESULTS

In the preterm birth group, methylation of and genes was significantly lower ( gene: 60.30% vs. 73.16%, <0.05; gene: 64.59% vs. 73.16%, <0.05), and the expression of the genes was significantly higher than that in the full-term group ( gene: 14.20 vs. 1.57, <0.01; gene: 1.18 vs. 10.34, <0.05).

CONCLUSION

Methylation of the and genes in the amnion was associated with preterm birth. Thus, and gene methylation status in the amnion may be valuable in explaining the mechanism underlying preterm birth.