Sobhy Nader M, Bayoumi Yasmin H, Mor Sunil K, El-Zahar Heba I, Goyal Sagar M
Department of Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Sharkia 44511, Egypt.
Department of Veterinary Population Medicine (Virology), University of Minnesota, St. Paul, MN 55108, USA.
Int J Vet Sci Med. 2018 Feb 9;6(1):22-30. doi: 10.1016/j.ijvsm.2018.02.001. eCollection 2018 Jun.
Foot and mouth disease virus (FMDV) was isolated from sloughed tongue epithelium of Egyptian cattle presenting with mouth lesions and ropy salivation in two Egyptian governorates (El-Fayoum and Dakahlia). The virus was isolated in Madin-Darby bovine kidney (MDBK) cells and identified by reverse transcription-polymerase chain reaction (RT-PCR). The complete genome was obtained by next generation sequencing. The strains isolated from El-Fayoum and Dakahlia were serotype A and O, respectively and both isolates had identity with the previously reported Egyptian strains. This study reports successive outbreaks of FMDV that occurred in Egypt during 2015-2016 and describes the dynamics of two outbreaks in addition to the use of cardiac biomarkers in the diagnosis of FMD-related myocarditis in calves and its clinical relevance. Serum cardiac troponin1 (cTn I) and creatinine kinase myocardial band (CK-MB) were measured. Mean serum cardiac troponin1 (cTn I) showed significant increase ( < 0.001) in FMDV-infected calves. The increase in fatal and recovered cases was (2.794 ± 0.502 ng/mL) and (1.196 ± 0.443 ng/mL), respectively, compared to the healthy control cases (0.014 ± 0.002 ng/mL). Thus, the serum cTn-I successfully diagnosed FMD-associated myocarditis in calves but not prognostic for the fatal cases. The FMDV sequences described in this study should further help in studying FMDV endemicity in Egypt, tracking the source of infection, selection of control strategies and vaccine updates. The study also determines the clinical relevance of cardiac biomarkers in diagnosis of FMDV-related myocarditis in infected calves.
口蹄疫病毒（FMDV）是从埃及两个省份（法尤姆省和达卡利亚省）出现口腔病变及流涎症状的病牛脱落的舌上皮中分离得到的。该病毒在马-达二氏牛肾（MDBK）细胞中分离培养，并通过逆转录-聚合酶链反应（RT-PCR）进行鉴定。通过下一代测序获得了完整基因组。从法尤姆省和达卡利亚省分离出的毒株分别为A型和O型，且这两种分离株与先前报道的埃及毒株具有一致性。本研究报告了2015 - 2016年期间埃及发生的口蹄疫病毒连续疫情，并描述了两次疫情的动态情况，此外还探讨了心脏生物标志物在诊断犊牛口蹄疫相关心肌炎中的应用及其临床意义。检测了血清心肌肌钙蛋白I（cTn I）和肌酸激酶同工酶（CK-MB）。口蹄疫病毒感染的犊牛血清心肌肌钙蛋白I（cTn I）平均水平显著升高（P<0.001）。与健康对照病例（0.014±0.002 ng/mL）相比，死亡病例和康复病例的cTn I升高水平分别为（2.794±0.502 ng/mL）和（1.196±0.443 ng/mL）。因此，血清cTn-I成功诊断了犊牛口蹄疫相关心肌炎，但对死亡病例不具有预后价值。本研究中描述的口蹄疫病毒序列应有助于进一步研究埃及口蹄疫病毒的地方性流行情况、追踪感染源、选择控制策略以及更新疫苗。该研究还确定了心脏生物标志物在诊断感染犊牛口蹄疫病毒相关心肌炎中的临床意义。
