Slaoui Mohamed, Fiette Laurence
Disposition, Safety and Animal Research, Sanofi-Aventis R&D, Vitry-sur-Seine, France.
Methods Mol Biol. 2011;691:69-82. doi: 10.1007/978-1-60761-849-2_4.
Histological procedures aim to provide good quality sections that can be used for a light microscopic evaluation of human or animal tissue changes in either spontaneous or induced diseases. Routinely, tissues are fixed with neutral formalin 10%, embedded in paraffin, and then manually sectioned with a microtome to obtain 4-5 μm-thick paraffin sections. Dewaxed sections are then stained with hematoxylin and eosin (H&E) or can be used for other purposes (special stains, immunohistochemistry, in situ hybridization, etc.). During this process, many steps and procedures are critical to ensure standard and interpretable sections. Key recomendations are given here to achieve this objective.
组织学程序旨在提供高质量的切片，可用于对人类或动物在自发或诱发疾病中的组织变化进行光学显微镜评估。通常，组织用10%中性福尔马林固定，石蜡包埋，然后用切片机手动切片，以获得4-5微米厚的石蜡切片。脱蜡后的切片再用苏木精和伊红（H&E）染色，或者可用于其他目的（特殊染色、免疫组织化学、原位杂交等）。在这个过程中，许多步骤和程序对于确保切片的标准性和可解释性至关重要。这里给出关键建议以实现这一目标。
