Ogura T, Mitsui T, Yamamoto I, Katayama E, Ota Z, Ogawa N
Life Sci. 1987 Jan 19;40(3):233-8. doi: 10.1016/0024-3205(87)90337-7.
To elucidate the role of atrial natriuretic peptide (ANP) and vasopressin (VP) in a hypertensive state, ANP and VP receptor bindings in spontaneously hypertensive rat (SHR) kidney were analyzed using the radiolabeled receptor assay (RRA) technique. Systolic blood pressure of SHR aged 12 weeks was statistically higher than that of age-matched Wistar Kyoto (WKY) rats. Maximum binding capacity (Bmax) of [125I]-ANP binding to the SHR kidney membrane preparations was statistically lower than that of WKY rats, but dissociation constant (Kd) was not significantly different. On the other hand, Bmax of [3H]-VP binding to the SHR kidney membrane preparations was statistically higher than that of WKY rats, but Kd were similar. Since the physiological action of ANP is natriuresis and VP is the most important antidiuretic hormone in mammalia, these opposite changes of ANP and VP receptor bindings in SHR kidney suggested that these peptides may play an important role in the pathophysiology of the hypertensive state, although it has not been confirmed as yet.
为阐明心钠素（ANP）和血管加压素（VP）在高血压状态中的作用，采用放射性标记受体分析法（RRA）技术分析了自发性高血压大鼠（SHR）肾脏中ANP和VP受体的结合情况。12周龄SHR的收缩压在统计学上高于年龄匹配的Wistar Kyoto（WKY）大鼠。[125I] - ANP与SHR肾膜制剂结合的最大结合容量（Bmax）在统计学上低于WKY大鼠，但解离常数（Kd）无显著差异。另一方面，[3H] - VP与SHR肾膜制剂结合的Bmax在统计学上高于WKY大鼠，但Kd相似。由于ANP的生理作用是利钠，而VP是哺乳动物中最重要的抗利尿激素，SHR肾脏中ANP和VP受体结合的这些相反变化表明，尽管尚未得到证实，但这些肽可能在高血压状态的病理生理学中起重要作用。
