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T偶数型噬菌体可通过与基因34(尾丝)或基因23(头部)重组来改变其宿主范围。

T-even type phages can change their host range by recombination with gene 34 (tail fibre) or gene 23 (head).

作者信息

Riede I

出版信息

Mol Gen Genet. 1986 Oct;205(1):160-3. doi: 10.1007/BF02428046.

Abstract

T-even type phages recognize their cellular receptors with the tip of their long tail fibres. The gene products involved in receptor recognition are proteins 37 and 38. While screening libraries of phage K3 with a probe of gene 38 from phage T2, a class of weakly hybridizing clones was found in addition to the expected clones of gene 38 of K3. One of these clones was identified as being from gene 23 of the phage which codes for the major head subunit; another clone originated from gene 34, which codes for the proximal half of the long tail fibres. Neither gene product 23 nor 34 is involved in receptor recognition. Phages can recombine with the DNA of the gene 23 and gene 34 clones and change the host range.

摘要

T偶数型噬菌体通过其长尾丝的末端识别细胞受体。参与受体识别的基因产物是蛋白质37和38。在用噬菌体T2的基因38探针筛选噬菌体K3文库时,除了预期的K3基因38克隆外,还发现了一类弱杂交克隆。其中一个克隆被鉴定为来自噬菌体的基因23,该基因编码主要头部亚基;另一个克隆来自基因34,该基因编码长尾丝的近端部分。基因产物23和34都不参与受体识别。噬菌体可以与基因23和基因34克隆的DNA重组并改变宿主范围。

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