Parker M L, Christensen A C, Boosman A, Stockard J, Young E T, Doermann A H
J Mol Biol. 1984 Dec 15;180(3):399-416. doi: 10.1016/0022-2836(84)90019-6.
We have determined the nucleotide sequence of gene 23 of bacteriophage T4 by the methods of Maxam and Gilbert and of Sanger. The identities of approximately 80% of the amino acid residues of the major capsid protein which is encoded by gene 23 were determined additionally by Edman degradation of the intact protein and its peptides. Fifteen gene 23 amber mutation sites have been located within the sequence, and the 3' transcription termination site for genes 21, 22 and 23 has been identified.
我们采用马克萨姆和吉尔伯特法以及桑格法测定了噬菌体T4基因23的核苷酸序列。通过对完整蛋白质及其肽段进行埃德曼降解，还确定了由基因23编码的主要衣壳蛋白约80%的氨基酸残基的序列。在该序列中已定位了15个基因23琥珀突变位点，并确定了基因21、22和23的3'转录终止位点。
