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微碎片化的人类脂肪组织与酶消化的基质血管部分相比，具有更高的周细胞含量和分泌活性。

Higher Pericyte Content and Secretory Activity of Microfragmented Human Adipose Tissue Compared to Enzymatically Derived Stromal Vascular Fraction.

机构信息

MRC Center for Regenerative Medicine, University of Edinburgh, Edinburgh, United Kingdom.

Image Institute, Milan, Italy.

出版信息

Stem Cells Transl Med. 2018 Dec;7(12):876-886. doi: 10.1002/sctm.18-0051. Epub 2018 Sep 26.

DOI:10.1002/sctm.18-0051
PMID:30255987
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6265639/
Abstract

Autologous adipose tissue is used for tissue repletion and/or regeneration as an intact lipoaspirate or as enzymatically derived stromal vascular fraction (SVF), which may be first cultured into mesenchymal stem cells (MSCs). Alternatively, transplant of autologous adipose tissue mechanically fragmented into submillimeter clusters has recently showed remarkable efficacy in diverse therapeutic indications. To document the biologic basis of the regenerative potential of microfragmented adipose tissue, we first analyzed the distribution of perivascular presumptive MSCs in adipose tissue processed with the Lipogems technology, observing a significant enrichment in pericytes, at the expense of adventitial cells, as compared to isogenic enzymatically processed lipoaspirates. The importance of MSCs as trophic and immunomodulatory cells, due to the secretion of specific factors, has been described. Therefore, we investigated protein secretion by cultured adipose tissue clusters or enzymatically derived SVF using secretome arrays. In culture, microfragmented adipose tissue releases many more growth factors and cytokines involved in tissue repair and regeneration, noticeably via angiogenesis, compared to isogenic SVF. Therefore, we suggest that the efficient tissue repair/regeneration observed after transplantation of microfragmented adipose tissue is due to the secretory ability of the intact perivascular niche. Stem Cells Translational Medicine 2018;7:876-886.

摘要

自体脂肪组织被用于组织填充和/或再生，可作为完整的脂肪抽吸物或作为酶衍生的基质血管部分 (SVF)，其可首先培养为间充质干细胞 (MSCs)。或者，最近将自体脂肪组织机械性地分割成亚毫米大小的簇的移植已在多种治疗适应症中显示出显著的疗效。为了记录微碎化脂肪组织的再生潜力的生物学基础，我们首先分析了用 Lipogems 技术处理的脂肪组织中血管周假定 MSC 的分布，与同种酶处理的脂肪抽吸物相比，发现周细胞明显富集，而 adventitial 细胞减少。由于特定因子的分泌，MSCs 作为营养和免疫调节细胞的重要性已经得到描述。因此，我们使用分泌组芯片研究了培养的脂肪组织簇或酶衍生的 SVF 的蛋白分泌。在培养中，与同种 SVF 相比，微碎化的脂肪组织释放出更多参与组织修复和再生的生长因子和细胞因子，特别是通过血管生成。因此，我们认为微碎化脂肪组织移植后观察到的有效组织修复/再生是由于完整的血管周龛的分泌能力。《干细胞转化医学》2018 年；7:876-886。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38db/6265639/f1538ec2aa0f/SCT3-7-876-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38db/6265639/25f983e62021/SCT3-7-876-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38db/6265639/b2fa99ecd1d5/SCT3-7-876-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38db/6265639/660c433515b4/SCT3-7-876-g003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38db/6265639/6dfbcfcd9e50/SCT3-7-876-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38db/6265639/fe3c9593981e/SCT3-7-876-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38db/6265639/f1538ec2aa0f/SCT3-7-876-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38db/6265639/25f983e62021/SCT3-7-876-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38db/6265639/b2fa99ecd1d5/SCT3-7-876-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38db/6265639/660c433515b4/SCT3-7-876-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38db/6265639/d950929d045d/SCT3-7-876-g004.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38db/6265639/fe3c9593981e/SCT3-7-876-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38db/6265639/f1538ec2aa0f/SCT3-7-876-g007.jpg

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