Lampalo Marina, Jukić Irena, Bingulac-Popović Jasna, Safić Hana, Ferara Nikola, Popović-Grle Sanja
Jordanovac University Department for Lung Diseases, Zagreb University Hospital Centre, Zagreb, Croatia.
Croatian Institute of Transfusion Medicine, Zagreb, Croatia; School of Medicine, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia.
Acta Clin Croat. 2018 Mar;57(1):96-102. doi: 10.20471/acc.2018.57.01.11.
Asthma is a chronic inflammatory disease that is characterized by reversible obstruction of airways, bronchial hyper-reactivity and airway remodeling. The etiology of asthma is multifactorial, with inheritance playing an important role. The aim of our study was to investigate the importance of biomarkers of asthma and the role of plasminogen activator inhibitor-1 (PAI-1) gene as a genetic factor that could be involved in the pathogenesis of asthma. The research was conducted at Jordanovac University Department for Lung Diseases and Croatian Institute of Transfusion Medicine. The research included 149 patients with asthma and 89 healthy individuals. We collected demographic data of both study groups, determined asthma severity using GINA guidelines, and the values of biomarkers and PAI-1 by using laboratory techniques. Based on the results, we concluded that patients with allergic phenotype of asthma were younger, had better lung function and higher levels of IgE. By observing FeNO values, we were not able to distinguish asthmatic patients that had been diagnosed with obstruction of airways from asthmatic patients with normal lung function because FeNO indicates the inflammatory component of disease. The 4G/5G polymorphism of PAI-1 gene did not show any statistically significant difference in the distribution of 4G/4G, 4G/5G and 5G/5G between the group of asthmatic patients and control group.
哮喘是一种慢性炎症性疾病，其特征为气道可逆性阻塞、支气管高反应性和气道重塑。哮喘的病因是多因素的，遗传起重要作用。我们研究的目的是调查哮喘生物标志物的重要性以及纤溶酶原激活物抑制剂-1（PAI-1）基因作为可能参与哮喘发病机制的遗传因素的作用。该研究在乔丹诺瓦茨大学肺病科和克罗地亚输血医学研究所进行。研究包括149例哮喘患者和89名健康个体。我们收集了两个研究组的人口统计学数据，使用全球哮喘防治创议（GINA）指南确定哮喘严重程度，并通过实验室技术测定生物标志物和PAI-1的值。根据结果，我们得出结论，具有过敏表型的哮喘患者更年轻，肺功能更好且IgE水平更高。通过观察呼出气一氧化氮（FeNO）值，我们无法区分已诊断为气道阻塞的哮喘患者和肺功能正常的哮喘患者，因为FeNO反映的是疾病的炎症成分。PAI-1基因的4G/5G多态性在哮喘患者组和对照组之间的4G/4G、4G/5G和5G/5G分布上未显示任何统计学显著差异。
