Lampalo Marina, Jukic Irena, Bingulac-Popovic Jasna, Marunica Ivona, Petlevski Roberta, Pavlisa Gordana, Popovic-Grle Sanja
Clinical Department for Lung Diseases Jordanovac, The University Hospital Centre Zagreb, Jordanovac Street 104, 10000, Zagreb, Croatia.
Croatian Institute of Transfusion Medicine, Zagreb, Croatia.
Eur Arch Otorhinolaryngol. 2017 Jun;274(6):2613-2619. doi: 10.1007/s00405-017-4502-2. Epub 2017 Mar 3.
Plasminogen activator inhibitor-1 (PAI-1) is a glycoprotein which has a role in tissue remodelling after inflammatory processes. The objective is to investigate the frequency of PAI-1 gene polymorphism (4G/5G) in patients with a lung ventilation dysfunction in asthma and allergic rhinitis. Genomic DNA was isolated and genotypes of polymorphism of PAI-1 4G/5G and ABO were determined using the methods of RT-PCR and PCR-SSP. Study group includes 145 adult patients diagnosed with chronic asthma, with all clinically relevant parameters and the laboratory markers of pO, IgE and eosinophils in sputum and nasal swab. In the processing of data, appropriate statistical tests (Kolmogorov-Smirnov test, median, interquartile ranges, χ and Mann-Whitney U tests) were used. Patients with symptoms of allergic rhinitis were significantly younger and had an almost four time higher levels of IgE (P = 0.001), higher pO (P = 0.002) and PEF (P = 0.036), compared to those who do not have these symptoms. Genotype PAI 4G/4G is significantly more common in patients with allergic rhinitis (28.1% vs. 16.1%; P = 0.017) compared to the genotype 5G/5G. Carriers of the genotype 4G/5G also have a borderline statistical significance. There were no statistically significant difference in the incidence of allergic rhinitis in the carriers of any ABO genotypes. The frequency of PAI genotype 4G/4G is significantly more common in patients with allergic rhinitis. The results suggest that the carriers of at least one 4G allele are at a higher risk for developing symptoms of allergic rhinitis in asthma.
纤溶酶原激活物抑制剂-1（PAI-1）是一种糖蛋白，在炎症过程后的组织重塑中发挥作用。目的是研究哮喘和过敏性鼻炎患者肺通气功能障碍时PAI-1基因多态性（4G/5G）的频率。采用RT-PCR和PCR-SSP方法分离基因组DNA，测定PAI-1 4G/5G和ABO多态性的基因型。研究组包括145例诊断为慢性哮喘的成年患者，记录所有临床相关参数以及痰液和鼻拭子中pO、IgE和嗜酸性粒细胞的实验室指标。在数据处理过程中，使用了适当的统计检验（Kolmogorov-Smirnov检验、中位数、四分位间距、χ检验和Mann-Whitney U检验）。与没有这些症状的患者相比，有过敏性鼻炎症状的患者明显更年轻，IgE水平几乎高四倍（P = 0.001），pO更高（P = 0.002），PEF更高（P = 0.036）。与5G/5G基因型相比，PAI 4G/4G基因型在过敏性鼻炎患者中明显更常见（28.1%对16.1%；P = 0.017）。4G/5G基因型携带者也具有临界统计学意义。任何ABO基因型携带者中过敏性鼻炎的发病率没有统计学显著差异。PAI基因型4G/4G在过敏性鼻炎患者中明显更常见。结果表明，至少携带一个4G等位基因的携带者在哮喘中发生过敏性鼻炎症状的风险更高。
