Knecht D A, Cohen S M, Loomis W F, Lodish H F
Mol Cell Biol. 1986 Nov;6(11):3973-83. doi: 10.1128/mcb.6.11.3973-3983.1986.
The Dictyostelium discoideum genome contains an estimated 17 to 20 actin genes. We report the identification of a new member of this multigene family, actin 15, and its complete nucleotide sequence and transcription initiation sites. We constructed transformation vectors carrying either the actin 15 promoter fused to the neomycin phosphotransferase gene from transposon Tn903 or the actin 6 promoter fused to the neomycin phosphotransferase gene from Tn5. Cells transformed with the actin 15 vector carried less than five copies of vector DNA, while cells transformed with the actin 6 vector carried more than 200 copies. In both cases, the vector appeared to be integrated into the chromosome as a tandem array. Gene fusion RNAs transcribed from the actin 15 and actin 6 vectors were regulated like endogenous actin genes during D. discoideum development. DNA sequences required for temporal and cell type-specific regulation of these genes were contained within 2.8 kilobases of 5' noncoding DNA for actin 15 and 0.7 kilobases of 5' noncoding DNA for actin 6.
盘基网柄菌基因组估计包含17至20个肌动蛋白基因。我们报告了这个多基因家族一个新成员肌动蛋白15的鉴定及其完整的核苷酸序列和转录起始位点。我们构建了转化载体，其中一个载体携带与转座子Tn903的新霉素磷酸转移酶基因融合的肌动蛋白15启动子，另一个载体携带与Tn5的新霉素磷酸转移酶基因融合的肌动蛋白6启动子。用肌动蛋白15载体转化的细胞携带少于五个拷贝的载体DNA，而用肌动蛋白6载体转化的细胞携带超过200个拷贝。在这两种情况下，载体似乎都以串联阵列的形式整合到染色体中。在盘基网柄菌发育过程中，从肌动蛋白15和肌动蛋白6载体转录的基因融合RNA的调控方式与内源性肌动蛋白基因相同。这些基因的时间和细胞类型特异性调控所需的DNA序列，肌动蛋白15位于5'非编码DNA的2.8千碱基内，肌动蛋白6位于5'非编码DNA的0.7千碱基内。
