Ng R, Abelson J
Proc Natl Acad Sci U S A. 1980 Jul;77(7):3912-6. doi: 10.1073/pnas.77.7.3912.
The yeast Saccharomyces cerevisiae is known to contain the highly conserved and unbiquitous protein actin. We have used cloned actin sequences from Dictyostelium discoideum to identify and clone the actin gene in yeast. Hybridization to genomic fragments of yeast DNA suggest that there is a single actin gene in yeast. We have determined the nucleotide sequence of that gene and its flanking regions. The sequence of the gene reveals an intervening sequence of 309 base pairs in the coding sequences at the 5' end of the gene. The existence and location of the intervening sequence was verified by using the dideoxy chain termination technique to determine the sequence at the 5' terminus of the actin mRNA. The similarity of the splice junction sequences in this gene to those found in higher eukaryotes suggests that yeast must possess a similar splicing enzyme.
已知酵母酿酒酵母中含有高度保守且普遍存在的蛋白质肌动蛋白。我们利用从盘基网柄菌克隆的肌动蛋白序列来鉴定和克隆酵母中的肌动蛋白基因。与酵母DNA基因组片段的杂交表明酵母中存在单个肌动蛋白基因。我们已经确定了该基因及其侧翼区域的核苷酸序列。该基因的序列显示在基因5'端的编码序列中有一个309个碱基对的间隔序列。通过使用双脱氧链终止技术确定肌动蛋白mRNA 5'末端的序列，验证了间隔序列的存在和位置。该基因中剪接连接序列与高等真核生物中发现的序列的相似性表明酵母必定拥有类似的剪接酶。
