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活性X染色体DNA在磷酸甘油酸激酶基因5'端富含鸟嘌呤加胞嘧啶的岛中聚集的八个CCGG位点处未发生甲基化。

Active X chromosome DNA is unmethylated at eight CCGG sites clustered in a guanine-plus-cytosine-rich island at the 5' end of the gene for phosphoglycerate kinase.

作者信息

Keith D H, Singer-Sam J, Riggs A D

出版信息

Mol Cell Biol. 1986 Nov;6(11):4122-5. doi: 10.1128/mcb.6.11.4122-4125.1986.

DOI:10.1128/mcb.6.11.4122-4125.1986
PMID:3025634
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC367182/
Abstract

The 5' control region and first exon for human X-chromosome-linked phosphoglycerate kinase is contained in a G + C-rich island. We measured methylation at all HpaII sites in this 5' region of leukocyte DNA. By use of a blotting procedure that allows analysis of small DNA fragments, we found that the HpaII sites are entirely methylated when from an inactive X chromosome and entirely unmethylated when from an active one. In contrast, methylation of HpaII sites in more downstream regions of the gene is essentially the same in active and inactive X chromosomes.

摘要

人类X染色体连锁磷酸甘油酸激酶的5'调控区和第一个外显子包含在一个富含G+C的岛中。我们测定了白细胞DNA该5'区域所有HpaII位点的甲基化情况。通过使用一种允许分析小DNA片段的印迹方法，我们发现当HpaII位点来自失活的X染色体时完全甲基化，而来自活性X染色体时则完全未甲基化。相比之下，该基因更下游区域的HpaII位点甲基化在活性和失活X染色体中基本相同。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/417e/367182/3c5fefce8f23/molcellb00095-0568-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/417e/367182/e3c50e8da26a/molcellb00095-0567-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/417e/367182/37302fdbbe71/molcellb00095-0567-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/417e/367182/3c5fefce8f23/molcellb00095-0568-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/417e/367182/e3c50e8da26a/molcellb00095-0567-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/417e/367182/37302fdbbe71/molcellb00095-0567-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/417e/367182/3c5fefce8f23/molcellb00095-0568-a.jpg

相似文献

1
Active X chromosome DNA is unmethylated at eight CCGG sites clustered in a guanine-plus-cytosine-rich island at the 5' end of the gene for phosphoglycerate kinase.活性X染色体DNA在磷酸甘油酸激酶基因5'端富含鸟嘌呤加胞嘧啶的岛中聚集的八个CCGG位点处未发生甲基化。
Mol Cell Biol. 1986 Nov;6(11):4122-5. doi: 10.1128/mcb.6.11.4122-4125.1986.
2
Differences in methylation on the active and inactive human X chromosomes.人类活性和非活性X染色体上的甲基化差异。
Ann Hum Genet. 1985 May;49(2):115-27. doi: 10.1111/j.1469-1809.1985.tb01683.x.
3
In vivo footprint and methylation analysis by PCR-aided genomic sequencing: comparison of active and inactive X chromosomal DNA at the CpG island and promoter of human PGK-1.通过PCR辅助基因组测序进行体内足迹和甲基化分析：人类磷酸甘油酸激酶-1（PGK-1）的CpG岛和启动子处活性与非活性X染色体DNA的比较
Genes Dev. 1990 Aug;4(8):1277-87. doi: 10.1101/gad.4.8.1277.
4
Demethylation of specific sites in the 5' region of the inactive X-linked human phosphoglycerate kinase gene correlates with the appearance of nuclease sensitivity and gene expression.失活的X连锁人类磷酸甘油酸激酶基因5'区域特定位点的去甲基化与核酸酶敏感性的出现及基因表达相关。
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5
High-resolution methylation analysis of the human hypoxanthine phosphoribosyltransferase gene 5' region on the active and inactive X chromosomes: correlation with binding sites for transcription factors.人类次黄嘌呤磷酸核糖基转移酶基因5'区域在活性和非活性X染色体上的高分辨率甲基化分析：与转录因子结合位点的相关性
Mol Cell Biol. 1994 Feb;14(2):1419-30. doi: 10.1128/mcb.14.2.1419-1430.1994.
6
DNA binding factors for the CpG-rich island containing the promoter of the human X-linked PGK gene.包含人类X连锁磷酸甘油酸激酶（PGK）基因启动子的富含CpG岛的DNA结合因子。
Somat Cell Mol Genet. 1988 Sep;14(5):461-72. doi: 10.1007/BF01534712.
7
Methylation analysis by genomic sequencing of 5' region of mouse Pgk-1 gene and a cautionary note concerning the method.通过对小鼠Pgk-1基因5'区域进行基因组测序的甲基化分析及关于该方法的警示说明
Somat Cell Mol Genet. 1993 Nov;19(6):529-41. doi: 10.1007/BF01233380.
8
An X chromosome inactivation assay based on differential methylation of a CpG island coupled to a VNTR polymorphism at the 5' end of the monoamine oxidase A gene.一种基于CpG岛差异甲基化与单胺氧化酶A基因5'端VNTR多态性相结合的X染色体失活检测方法。
Hum Mol Genet. 1992 Jun;1(3):187-94. doi: 10.1093/hmg/1.3.187.
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Use of a HpaII-polymerase chain reaction assay to study DNA methylation in the Pgk-1 CpG island of mouse embryos at the time of X-chromosome inactivation.在X染色体失活时，使用HpaII聚合酶链反应分析法研究小鼠胚胎Pgk-1 CpG岛中的DNA甲基化。
Mol Cell Biol. 1990 Sep;10(9):4987-9. doi: 10.1128/mcb.10.9.4987-4989.1990.
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5-Azacytidine-induced reactivation of the human X chromosome-linked PGK1 gene is associated with a large region of cytosine demethylation in the 5' CpG island.5-氮杂胞苷诱导的人类X染色体连锁PGK1基因的重新激活与5' CpG岛中一大片胞嘧啶去甲基化区域相关。
Proc Natl Acad Sci U S A. 1990 Jun;87(11):4174-8. doi: 10.1073/pnas.87.11.4174.

引用本文的文献

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Sci Rep. 2023 Aug 8;13(1):12856. doi: 10.1038/s41598-023-34413-3.
2
Rapid determination of clonality by detection of two closely-linked X chromosome exonic polymorphisms using allele-specific PCR.使用等位基因特异性PCR检测两个紧密连锁的X染色体外显子多态性快速确定克隆性
J Clin Invest. 1997 Apr 15;99(8):1984-90. doi: 10.1172/JCI119366.
3
In vivo footprinting and high-resolution methylation analysis of the mouse hypoxanthine phosphoribosyltransferase gene 5' region on the active and inactive X chromosomes.

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Methylation of the hypoxanthine phosphoribosyltransferase locus on the human X chromosome: implications for X-chromosome inactivation.人类X染色体上次黄嘌呤磷酸核糖基转移酶基因座的甲基化：对X染色体失活的影响
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Analysis of DNase 1 sensitivity and methylation of active and inactive X chromosomes of kangaroos (Macropus robustus) by in situ nick translation.通过原位缺口平移分析袋鼠（粗壮大袋鼠）活性和非活性X染色体的DNase 1敏感性及甲基化情况。
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High-resolution methylation analysis of the human hypoxanthine phosphoribosyltransferase gene 5' region on the active and inactive X chromosomes: correlation with binding sites for transcription factors.人类次黄嘌呤磷酸核糖基转移酶基因5'区域在活性和非活性X染色体上的高分辨率甲基化分析：与转录因子结合位点的相关性
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Progressive increases in the methylation status and heterochromatinization of the myoD CpG island during oncogenic transformation.在致癌转化过程中，肌分化抗原（MyoD）基因座控制区（CpG岛）的甲基化状态和异染色质化逐渐增加。
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DNA methylation stabilizes X chromosome inactivation in eutherians but not in marsupials: evidence for multistep maintenance of mammalian X dosage compensation.DNA甲基化在真兽类中稳定X染色体失活，但在有袋类中则不然：哺乳动物X染色体剂量补偿多步骤维持的证据。
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Mammalian X-chromosome inactivation.哺乳动物X染色体失活。
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Genomic sequencing.基因组测序
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DNA methylation and gene function.DNA甲基化与基因功能。
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