Minstry of health, Kafr El Shiekh General Hospital, Pharmacy department, Kafr El-Shaikh, Egypt.
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Cairo University, Kafr El Sheikh, Giza, Egypt.
J Biochem Mol Toxicol. 2018 Nov;32(11):e22218. doi: 10.1002/jbt.22218. Epub 2018 Sep 26.
Frequent consumption of fructose and saturated fatty acids increase risk of metabolic syndrome (MS). Features of MS include insulin resistance, dyslipidemia, visceral obesity, and hypertension. The aim of this study was to investigate the role of omega-3 and l-carnitine in ameliorating features of MS.
MS was induced in rats by high-fructose high-fat fed diet for 8 weeks. They were randomly divided into five groups: normal control, MS control group treated with saline, MS groups given omega-3 (260 mg/kg), l-carnitine (200 mg/kg), or metformin (100 mg/kg) daily for 4 weeks. Body weight, relative organ weight, glucose, insulin, adiponectin, and lipid profiles were estimated. Also glucose transporter 4 (GLUT4) content and peroxisome proliferator-activated receptor-gamma (PPARγ) protein expressions were determined.
Omega-3 and l-carnitine caused decrease in both MS-induced increase in body weight and glucose similar to metformin. They reduced insulin level and resistance with increased adiponectin, and correction of MS-induced hyperlipidemia. Drugs also increased GLUT4 and PPARγ protein expression compared with MS control group.
Omega-3 and l-carnitine improve features of MS via increased GLUT4 and PPARγ expression.
频繁摄入果糖和饱和脂肪酸会增加代谢综合征（MS）的风险。MS 的特征包括胰岛素抵抗、血脂异常、内脏肥胖和高血压。本研究旨在探讨 ω-3 和左旋肉碱在改善 MS 特征中的作用。
通过高果糖高脂肪饮食喂养 8 周诱导大鼠 MS。他们被随机分为五组：正常对照组、用生理盐水治疗的 MS 对照组、每日给予 ω-3（260mg/kg）、左旋肉碱（200mg/kg）或二甲双胍（100mg/kg）的 MS 组，持续 4 周。评估体重、相对器官重量、葡萄糖、胰岛素、脂联素和血脂谱。还测定了葡萄糖转运蛋白 4（GLUT4）含量和过氧化物酶体增殖物激活受体-γ（PPARγ）蛋白表达。
与二甲双胍类似，ω-3 和左旋肉碱可降低 MS 诱导的体重和葡萄糖增加，降低胰岛素水平和抵抗，增加脂联素，并纠正 MS 诱导的高脂血症。与 MS 对照组相比，药物还增加了 GLUT4 和 PPARγ 蛋白的表达。
ω-3 和左旋肉碱通过增加 GLUT4 和 PPARγ 的表达来改善 MS 的特征。
