Department of Biomedical Sciences, Elson S. Floyd College of Medicine, Washington State University, Spokane, WA 99202, USA.
Tissue Imaging and Proteomics Laboratory, Washington State University, Pullman, WA 99164, USA.
Int J Mol Sci. 2018 Sep 25;19(10):2912. doi: 10.3390/ijms19102912.
Porcine mammary fatty tissues represent an abundant source of natural biomaterial for generation of breast-specific extracellular matrix (ECM). Here we report the extraction of total ECM proteins from pig breast fatty tissues, the fabrication of hydrogel and porous scaffolds from the extracted ECM proteins, the structural properties of the scaffolds (tissue matrix scaffold, TMS), and the applications of the hydrogel in human mammary epithelial cell spatial cultures for cell surface receptor expression, metabolomics characterization, acini formation, proliferation, migration between different scaffolding compartments, and in vivo tumor formation. This model system provides an additional option for studying human breast diseases such as breast cancer.
猪乳腺脂肪组织是生成乳腺特异性细胞外基质（ECM）的天然生物材料的丰富来源。本研究从猪乳腺脂肪组织中提取 ECM 蛋白，用提取的 ECM 蛋白制备水凝胶和多孔支架，研究支架的结构特性（组织基质支架，TMS），并将水凝胶应用于人类乳腺上皮细胞的空间培养，以研究细胞表面受体表达、代谢组学特征、小腔形成、增殖、在不同支架隔室之间的迁移以及体内肿瘤形成。该模型系统为研究人类乳腺疾病（如乳腺癌）提供了另一种选择。
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