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新西兰昆布中提取的低分子量岩藻聚糖硫酸酯对 RAW264.7 巨噬细胞的免疫激活作用。

Immune Activation of RAW264.7 Macrophages by Low Molecular Weight Fucoidan Extracted from New Zealand Undaria pinnatifida.

机构信息

College of Life Sciences and Oceanography, Shenzhen Key Laboratory of Marine Bioresources and Ecology , Shenzhen University , Shenzhen 518060 , PR China.

School of Science and School of Interprofessional Health Studies, Faculty of Health and Environmental Sciences , Auckland University of Technology , Auckland 1142 , New Zealand.

出版信息

J Agric Food Chem. 2018 Oct 17;66(41):10721-10728. doi: 10.1021/acs.jafc.8b03698. Epub 2018 Oct 8.

Abstract

Fucoidan, a sulfated polysaccharide extracted from brown seaweeds, has been shown to possess various bioactivities. In particular, low molecular weight fucoidan (LMWF) has been shown to have better bioactivities. In this study, a LMWF (<10 kDa) was extracted from New Zealand Undaria pinnatifida and investigated for its immune modulation effects. LMWF at a concentration range from 1 to 50 μg/mL exerted an effective immune activation in RAW264.7 macrophages. LMWF treatment promoted significant NO release, iNOS expression, and TNF-α and IL-6 secretion in a concentration-dependent manner. It also significantly stimulated the activation of NF-κB and MAPK signaling pathways, and specific inhibitors of NF-κB and MAPK pathways diminished the stimulation, confirming the activation pathways. These results indicate that LMWF possesses potential health benefits through immune-stimulation, which may lead to future pharmaceutical development.

摘要

岩藻聚糖硫酸酯是从褐藻中提取的一种硫酸多糖,具有多种生物活性。特别是,低分子量岩藻聚糖(LMWF)具有更好的生物活性。本研究从新西兰昆布中提取了一种 LMWF(<10 kDa),并研究了其免疫调节作用。在 1 至 50 μg/mL 的浓度范围内,LMWF 对 RAW264.7 巨噬细胞发挥有效的免疫激活作用。LMWF 处理以浓度依赖性方式显著促进了 NO 的释放、iNOS 的表达以及 TNF-α和 IL-6 的分泌。它还显著刺激了 NF-κB 和 MAPK 信号通路的激活,并且 NF-κB 和 MAPK 通路的特异性抑制剂减弱了刺激,证实了激活途径。这些结果表明,LMWF 通过免疫刺激具有潜在的健康益处,这可能导致未来的药物开发。

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