Wang Zhikun, Yang Qiang, Shao Yupeng, Zhang Binbin, Feng Aiyun, Meng Fanli, Li Wenbin
Key Laboratory of Soybean Biology in Chinese Ministry of Education/Key Laboratory of Biology and Genetics & Breeding for Soybean in Northeast China, Ministry of Agriculture ( Northeast Agricultural University ), Harbin , China.
Acta Biol Hung. 2018 Sep;69(3):270-282. doi: 10.1556/018.68.2018.3.4.
Late embryonic proteins (LEA) gene family was abundant mainly in higher plant embryos, which could protect the embryos from the damage caused by abiotic stress, especially drought and salt stresses. In the present study, GmLEA2-1 was cloned from soybean leaf tissue treated by 10% polyethylene glycol 6000 (PEG6000). The results of quantitative real-time PCR (qRT-PCR) revealed a variety of expression patterns of GmLEA2-1 in various tissues of soybean (root, stem, leaf, flower, pod, early embryo and late embryo). GmLEA2-1 gene shared a lower sequence similarity with other typical LEA genes of same group from different species, but similar functions. Overexpression of GmLEA2-1 in transgenic Arabidopsis thaliana conferred tolerance to drought and salt stresses. The fresh weight and dry weight of seedling, the primary root length and the lateral root density of transgenic Arabidopsis plants were higher than those of wild type Arabidopsis (WT) under drought and salt stresses. Cis-acting regulatory elements in the GmLEA2-1 promoter were also predicted. These data demonstrate that GmLEA2-1 protein play an important role in improving drought and salt tolerance in plants.
晚期胚胎丰富蛋白（LEA）基因家族主要在高等植物胚胎中大量存在，其能够保护胚胎免受非生物胁迫，尤其是干旱和盐胁迫造成的损害。在本研究中，从经10%聚乙二醇6000（PEG6000）处理的大豆叶片组织中克隆得到GmLEA2-1。实时定量PCR（qRT-PCR）结果显示GmLEA2-1在大豆的各种组织（根、茎、叶、花、荚、早期胚胎和晚期胚胎）中呈现多种表达模式。GmLEA2-1基因与来自不同物种的同一组其他典型LEA基因的序列相似性较低，但功能相似。在转基因拟南芥中过表达GmLEA2-1可使其获得对干旱和盐胁迫的耐受性。在干旱和盐胁迫下，转基因拟南芥植株的幼苗鲜重和干重、主根长度和侧根密度均高于野生型拟南芥（WT）。还对GmLEA2-1启动子中的顺式作用调控元件进行了预测。这些数据表明GmLEA2-1蛋白在提高植物的耐旱性和耐盐性方面发挥着重要作用。
