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大豆晚期胚胎丰富蛋白：鉴定、表达分析及 GmLEA4_19 在干旱胁迫中的作用。

The Late Embryogenesis Abundant Proteins in Soybean: Identification, Expression Analysis, and the Roles of GmLEA4_19 in Drought Stress.

机构信息

Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou 225009, China.

Zhongshan Biological Breeding Laboratory, No. 50 Zhongling Street, Nanjing 210014, China.

出版信息

Int J Mol Sci. 2023 Oct 2;24(19):14834. doi: 10.3390/ijms241914834.

DOI:10.3390/ijms241914834
PMID:37834282
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10573439/
Abstract

Late embryogenesis abundant (LEA) proteins play important roles in regulating plant growth and responses to various abiotic stresses. In this research, a genome-wide survey was conducted to recognize the LEA genes in . A total of 74 GmLEA was identified and classified into nine subfamilies based on their conserved domains and the phylogenetic analysis. Subcellular localization, the duplication of genes, gene structure, the conserved motif, and the prediction of cis-regulatory elements and tissue expression pattern were then conducted to characterize GmLEAs. The expression profile analysis indicated that the expression of several GmLEAs was a response to drought and salt stress. The co-expression-based gene network analysis suggested that soybean LEA proteins may exert regulatory effects through the metabolic pathways. We further explored GnLEA4_19 function in and the results suggests that overexpressed GmLEA4_19 in increased plant height under mild or serious drought stress. Moreover, the overexpressed GmLEA4_19 soybean also showed a drought tolerance phenotype. These results indicated that GmLEA4_19 plays an important role in the tolerance to drought and will contribute to the development of the soybean transgenic with enhanced drought tolerance and better yield. Taken together, this study provided insight for better understanding the biological roles of genes in soybean.

摘要

晚期胚胎丰富蛋白（LEA）在调节植物生长和响应各种非生物胁迫方面发挥着重要作用。在这项研究中，进行了全基因组调查，以识别. 中的 LEA 基因。共鉴定出 74 个 GmLEA，并根据其保守结构域和系统发育分析将其分为九个亚家族。然后进行亚细胞定位、基因重复、基因结构、保守基序以及顺式调控元件和组织表达模式的预测，以表征 GmLEAs。表达谱分析表明，几个 GmLEAs 的表达是对干旱和盐胁迫的响应。基于共表达的基因网络分析表明，大豆 LEA 蛋白可能通过代谢途径发挥调节作用。我们进一步探索了 GnLEA4_19 在 中的功能，结果表明，在轻度或严重干旱胁迫下过表达 GmLEA4_19 的 株高增加。此外，过表达 GmLEA4_19 的大豆也表现出耐旱表型。这些结果表明，GmLEA4_19 在耐旱性中起着重要作用，将有助于开发具有增强耐旱性和更好产量的转基因大豆。总之，本研究为更好地理解大豆 基因的生物学功能提供了线索。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/354b/10573439/88ead30ee226/ijms-24-14834-g010.jpg
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