Aguilar-Montes de Oca Saúl, Montes-de-Oca-Jiménez Roberto, Carlos Vázquez-Chagoyán Juan, Barbabosa-Pliego Alberto, Eliana Rivadeneira-Barreiro Pilar, C Zambrano-Rodríguez Pablo
Centro de Investigación y Estudios Avanzados en Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Carretera Toluca-Atlacomulco, Km 15.5, Toluca 50200, CP, Mexico.
Departamento de Veterinaria, Facultad de Ciencias Veterinarias, Universidad Técnica de Manabí, Portoviejo 130105, Ecuador.
Vet Sci. 2022 Oct 12;9(10):561. doi: 10.3390/vetsci9100561.
Peptides constitute an alternative and interesting option to develop treatments, vaccines, and diagnostic tools as they demonstrate their scope in several health aspects; as proof of this, commercial peptides for humans and animals are available on the market and used daily. This review aimed to know the role of peptides in the field of veterinary diagnosis, and include peptide-based enzyme-linked immunosorbent assay (pELISA), lateral flow devices, and peptide latex agglutination tests that have been developed to detect several pathogens including viruses and bacteria of health and production relevance in domestic animals. Studies in cattle, small ruminants, dogs, cats, poultry, horses, and even aquatic organisms were reviewed. Different studies showed good levels of sensitivity and specificity against their target, moreover, comparisons with commercial kits and official tests were performed which allowed appraising their performance. Chemical synthesis, recombinant DNA technology, and enzymatic synthesis were reviewed as well as their advantages and drawbacks. In addition, we discussed the intrinsic limitations such as the small size or affinity to polystyrene membrane and mention several strategies to overcome these problems. The use of peptides will increase in the coming years and their utility for diagnostic purposes in animals must be evaluated.
肽是开发治疗方法、疫苗和诊断工具的一种替代且有趣的选择，因为它们在多个健康领域展现出了自身的应用范围；作为例证，市面上已有供人类和动物使用的商业化肽，并且人们每天都在使用。本综述旨在了解肽在兽医诊断领域的作用，包括已开发用于检测多种病原体（包括对家畜健康和生产具有重要意义的病毒和细菌）的基于肽的酶联免疫吸附测定（pELISA）、侧向流动装置和肽乳胶凝集试验。对牛、小型反刍动物、犬、猫、家禽、马甚至水生生物的相关研究进行了综述。不同研究显示出对其靶标的良好敏感性和特异性水平，此外，还与商业试剂盒和官方检测进行了比较，从而能够评估它们的性能。对化学合成、重组DNA技术和酶促合成及其优缺点也进行了综述。此外，我们讨论了诸如尺寸小或对聚苯乙烯膜的亲和力等内在局限性，并提及了几种克服这些问题的策略。未来几年肽的应用将会增加，必须对其在动物诊断中的效用进行评估。
