Department of Chemistry, Molecular Sciences Research Hub, Imperial College London, London, W12 0BZ, UK.
Institute of Chemical Biology, Molecular Sciences Research Hub, Imperial College London, London, W12 0BZ, UK.
Sci Rep. 2018 Sep 26;8(1):14380. doi: 10.1038/s41598-018-32706-6.
We present a simple, facile method to micropattern planar metal electrodes defined by the geometry of a microfluidic channel network template. By introducing aqueous solutions of metal into reversibly adhered PDMS devices by desiccation instead of flow, we are able to produce difficult to pattern "dead end" or discontinuous features with ease. We characterize electrodes fabricated using this method and perform electrical lysis of mammalian cancer cells and demonstrate their use as part of an antibody capture assay for GFP. Cell lysis in microwell arrays is achieved using the electrodes and the protein released is detected using an antibody microarray. We show how the template channels used as part of the workflow for patterning the electrodes may be produced using photolithography-free methods, such as laser micromachining and PDMS master moulding, and demonstrate how the use of an immiscible phase may be employed to create electrode spacings on the order of 25-50 μm, that overcome the current resolution limits of such methods. This work demonstrates how the rapid prototyping of electrodes for use in total analysis systems can be achieved on the bench with little or no need for centralized facilities.
我们提出了一种简单、便捷的方法，可以通过微流控通道网络模板的几何形状对平面金属电极进行微图案化。通过将金属水溶液通过干燥而不是流动引入到可逆附着的 PDMS 器件中，我们能够轻松地生成难以图案化的“死胡同”或不连续特征。我们对使用这种方法制造的电极进行了表征，并对哺乳动物癌细胞进行了电裂解实验，展示了它们在 GFP 抗体捕获测定中的应用。使用电极在微井阵列中实现细胞裂解，并使用抗体微阵列检测释放的蛋白质。我们展示了如何使用免光刻方法（例如激光微加工和 PDMS 主模具制造）来制作作为电极图案化工作流程一部分的模板通道，并演示了如何使用不混相来创建间距约为 25-50 μm 的电极，从而克服了此类方法的当前分辨率限制。这项工作展示了如何在台面上实现用于整体分析系统的电极的快速原型制作，而几乎不需要集中的设施。
