Sass P, Field J, Nikawa J, Toda T, Wigler M
Proc Natl Acad Sci U S A. 1986 Dec;83(24):9303-7. doi: 10.1073/pnas.83.24.9303.
A gene, PDE2, has been cloned from the yeast Saccharomyces cerevisiae that, when present in high copy, reverses the phenotypic effects of RAS2Val19, a mutant form of the RAS2 gene that renders yeast cells sensitive to heat shock and starvation. It has previously been shown that the RAS proteins are potent activators of yeast adenylate cyclase. We report here that PDE2 encodes a high-affinity cAMP phosphodiesterase that shares sequence homology with animal cell phosphodiesterases. These results therefore imply that the effects of RAS2Val19 are mediated through its changes in cAMP concentration.
已从酿酒酵母中克隆出一种基因PDE2，当它以高拷贝形式存在时，可逆转RAS2Val19的表型效应，RAS2Val19是RAS2基因的一种突变形式，会使酵母细胞对热休克和饥饿敏感。此前已有研究表明，RAS蛋白是酵母腺苷酸环化酶的有效激活剂。我们在此报告，PDE2编码一种高亲和力的cAMP磷酸二酯酶，它与动物细胞磷酸二酯酶具有序列同源性。因此，这些结果表明RAS2Val19的效应是通过其对cAMP浓度的改变来介导的。
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