Reddy E P, Reynolds R K, Santos E, Barbacid M
Nature. 1982 Nov 11;300(5888):149-52. doi: 10.1038/300149a0.
The genetic change that leads to the activation of the oncogene in T24 human bladder carcinoma cells is shown to be a single point mutation of guanosine into thymidine. This substitution results in the incorporation of valine instead of glycine as the twelfth amino acid residue of the T24 oncogene-encoded p21 protein. Thus, a single amino acid substitution appears to be sufficient to confer transforming properties on the gene product of the T24 human bladder carcinoma oncogene.
导致T24人膀胱癌细胞中癌基因激活的基因变化被证明是鸟苷到胸苷的单点突变。这种取代导致缬氨酸取代甘氨酸掺入T24癌基因编码的p21蛋白的第12个氨基酸残基中。因此，单个氨基酸取代似乎足以赋予T24人膀胱癌癌基因的基因产物转化特性。
