用于健康和圆锥角膜人眼角膜细胞结构成像的亚微米轴向分辨率光学相干断层扫描技术
Sub-micrometer axial resolution OCT for imaging of the cellular structure of healthy and keratoconic human corneas.
作者信息
Bizheva Kostadinka, Tan Bingyao, MacLelan Benjamin, Kralj Olivera, Hajialamdari Mojtaba, Hileeto Denise, Sorbara Luigina
机构信息
Department of Physics and Astronomy, University of Waterloo, Waterloo, ON, N2L3G1, Canada; School of Optometry and Vision Science, University of Waterloo, Waterloo, ON, N2L3G1, Canada; Systems Design Engineering Department, University of Waterloo, Waterloo, ON, N2L3G1, Canada.
Department of Physics and Astronomy, University of Waterloo, Waterloo, ON, N2L3G1, Canada.
出版信息
Biomed Opt Express. 2017 Jan 12;8(2):800-812. doi: 10.1364/BOE.8.000800. eCollection 2017 Feb 1.
Corneal degenerative conditions such as keratoconus (KC) cause progressive damage to the anterior corneal tissue and eventually severely compromise visual acuity. The ability to visualize corneal tissue damage at cellular or sub-cellular level at different stages of development of KC and other corneal diseases, can aid the early diagnostics as well as the development of more effective treatment approaches for various corneal pathologies, including keratoconus. Here, we present the optical design of an optical coherence tomography system that can achieve 0.95 µm axial resolution in biological tissue and provide test results for the system's spatial resolution and sensitivity. Corneal images acquired with this system from healthy and keratoconic human subjects reveal the cellular and sub-cellular structure of the corneal epithelium, as well as the normal and abnormal structure of the Bowman's membrane and the anterior corneal stroma.
诸如圆锥角膜（KC）之类的角膜退行性疾病会对角膜前部组织造成渐进性损害，并最终严重损害视力。在圆锥角膜和其他角膜疾病发展的不同阶段，在细胞或亚细胞水平上可视化角膜组织损伤的能力，有助于早期诊断以及开发针对包括圆锥角膜在内的各种角膜病变的更有效治疗方法。在此，我们展示了一种光学相干断层扫描系统的光学设计，该系统在生物组织中可实现0.95微米的轴向分辨率，并提供该系统空间分辨率和灵敏度的测试结果。使用该系统从健康和圆锥角膜患者身上获取的角膜图像，揭示了角膜上皮的细胞和亚细胞结构，以及Bowman膜和角膜前基质的正常和异常结构。
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