He Xiao-Feng, Zhang Hui-Jie, Cao Jin-Gui, Liu Fang, Wang Jian-Kang, Ma Wen-Jie, Yin Wen
Department of Infection Control, Air Force General Hospital, Beijing 100142, China.
Ministry of Education Key Laboratory of Diagnostic Medicine, College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.
Genes Dis. 2017 Jul 8;4(3):163-169. doi: 10.1016/j.gendis.2017.07.001. eCollection 2017 Sep.
In clinical practice, the important hygienic prevention of bacterial pathogen spread is disinfection of potentially contaminated area. Benzalkonium bromide and chlorhexidine acetate are commonly used disinfectants with a broad spectrum of anti-microbial effect. It is vital to inhibit the spread of pathogen in hospital. However, a large number of pathogens with the decreased antiseptic susceptibility have been isolated from clinical samples which showed an increased minimal inhibitory concentration (MIC) against those antiseptics. These resistant pathogens are the major causes for nosocomial cross-infections in hospital. The present study demonstrated the utility of Oxford plate assay system in determining the potential disinfectant resistance of bacteria. The microbiological assay is based on the inhibitory effect of tested disinfectants upon the strains of and . Statistical analysis of the bioassay results indicated the linear correlation (r = 0.87-0.99, < 0.01) between the diameter of growth inhibition zone and the log dosage of the tested disinfectants. Moreover, comparison of inhibitory efficacy of benzalkonium bromide upon 29 strains isolated from clinical samples by both Oxford plate method and broth dilution method showed that the diameter of growth inhibition zone has significantly negative correlation with the minimal inhibitory concentration (MIC) (r = -0.574, < 0.001). These results suggest that the Oxford plate is a simple and time-saving method in detecting potential clinical disinfectant resistance and its usefulness for routine surveillance of pathogenic resistance to disinfectants warrants further investigation.
在临床实践中，预防细菌病原体传播的重要卫生措施是对潜在污染区域进行消毒。苯扎溴铵和醋酸氯己定是常用的具有广谱抗菌作用的消毒剂。抑制病原体在医院内传播至关重要。然而，从临床样本中分离出了大量对防腐剂敏感性降低的病原体，这些病原体对那些防腐剂的最低抑菌浓度（MIC）有所增加。这些耐药病原体是医院内医院感染的主要原因。本研究证明了牛津平板测定系统在确定细菌潜在消毒剂耐药性方面的实用性。微生物测定基于受试消毒剂对 和 菌株的抑制作用。生物测定结果的统计分析表明，生长抑制圈直径与受试消毒剂的对数剂量之间存在线性相关性（r = 0.87 - 0.99，< 0.01）。此外，通过牛津平板法和肉汤稀释法比较苯扎溴铵对从临床样本中分离出的29株菌株的抑制效果，结果表明生长抑制圈直径与最低抑菌浓度（MIC）呈显著负相关（r = -0.574，< 0.001）。这些结果表明，牛津平板法是一种检测潜在临床消毒剂耐药性的简单且省时的方法，其在病原体对消毒剂耐药性常规监测中的实用性值得进一步研究。
