AlTuraifi Fatimah Hejji, AlMomatin Ali A, Badger-Emeka Lorina, Emeka Promise Madu, Islam Mohammed Monirul
College of Clinical Pharmacy, King Faisal University, Al-Ahsa, Saudi Arabia.
Department of Biomedical Sciences, College of Medicine, King Faisal University, Al-Ahsa, Saudi Arabia.
Environ Health Insights. 2019 Nov 7;13:1178630219887393. doi: 10.1177/1178630219887393. eCollection 2019.
Water recreational facilities like swimming pools attract people of all ages. However, these facilities are very suitable for the transmission of various microbial diseases and have been shown to pose public health concerns.
This study assesses the presence of different Gram-negative bacteria pathogens and their antimicrobial susceptibility pattern in both private and public pools in Al-Ahsa.
11 private and 3 public recreational water facilities were sampled for the study. Collected water samples were inoculated into nutrient broth and incubated aerobically for 24 hours. The overnight growth was plated out on blood and MacConkey agars. Pure cultures of the bacteria samples were used for identification and antimicrobial susceptibility test using the Vitek 2 compactautomated system (BioMerieux, Marcy L'Etoile, France). Minimum inhibitory concentration was also provided by the Vitek 2 compact automated system.
13 different Gram-negative bacteria species isolates were encountered in both pool types sampled. More of potential pathogens were isolated from the private than the public pools, of which and constituted 50% and 43%, respectively, of all the isolates. Findings also revealed a varied minimum inhibitory concentrations (MICs) indicating that the isolates were of different strains. Antibiotic susceptibility pattern also showed variability among the isolates.
This study has revealed a potential health risk associated with the use of water recreational facilities. The presence of and suggests a public health concern and should be looked into.
游泳池等水上娱乐设施吸引着各年龄段的人群。然而，这些设施非常适合各种微生物疾病的传播，并且已被证明会引发公共卫生问题。
本研究评估了沙特东部省私人和公共游泳池中不同革兰氏阴性菌病原体的存在情况及其抗菌药敏模式。
本研究对11个私人和3个公共娱乐用水设施进行了采样。将采集的水样接种到营养肉汤中，需氧培养24小时。将过夜培养物接种到血琼脂和麦康凯琼脂平板上。使用Vitek 2 compact自动化系统（法国生物梅里埃公司，马西莱图瓦勒）对细菌样本的纯培养物进行鉴定和抗菌药敏试验。Vitek 2 compact自动化系统还提供了最低抑菌浓度。
在两种类型的采样游泳池中均发现了13种不同的革兰氏阴性菌分离株。从私人游泳池中分离出的潜在病原体比公共游泳池更多，其中[具体菌种1]和[具体菌种2]分别占所有分离株的50%和43%。研究结果还显示了不同的最低抑菌浓度（MICs），表明这些分离株属于不同的菌株。抗生素药敏模式在分离株之间也表现出差异。
本研究揭示了使用水上娱乐设施存在的潜在健康风险。[具体菌种1]和[具体菌种2]的存在表明存在公共卫生问题，应予以关注。
