Department of Environmental Science, Chongqing University, Chongqing, 400044, People's Republic of China.
State Key Laboratory of Coal Mine Disaster Dynamics and Control, Chongqing University, Chongqing, 400044, People's Republic of China.
Biodegradation. 2018 Dec;29(6):567-577. doi: 10.1007/s10532-018-9853-x. Epub 2018 Sep 26.
A previous study demonstrated that denitrification synergized with Anammox could accelerate the anaerobic degradation of benzene. The inhibitory effects of benzene, toluene, phenol and benzoate in single and combination on Anammox activity were investigated by short-term batch tests. The results indicated that the inhibition of single compounds on Anammox could be well fitted with the extended non-competitive and Luong inhibition kinetic models. The inhibitions of the individual compound were in order as follows: benzene > toluene > phenol > benzoate. The joint inhibitions of bi-component mixtures of benzene with toluene, benzene with phenol and benzene with benzoate on Anammox activity were additive; the joint inhibition of a tri-component mixture (benzene, toluene and phenol) was partly additive; and the joint inhibition of a multicomponent mixture (benzene, toluene, phenol and benzoate) was synergistic. The effect of benzoate on the denitrification-Anammox synergy for benzene degradation was evaluated using a long-term test. Although the average rate of benzene degradation decreased by 13% with the addition of 10 mg L benzoate, the average rate of NO and NH increased by approximately 1- and 0.56-fold, respectively, suggesting that benzoate favors the stability of the denitrification-Anammox synergy. The carboxylation of benzene would be a more favorable pathway for the anaerobic degradation of benzene under denitrification synergized with Anammox.
先前的研究表明，反硝化协同厌氧氨氧化可以加速苯的厌氧降解。通过短期批式实验，考察了苯、甲苯、苯酚和苯甲酸在单一和组合条件下对厌氧氨氧化活性的抑制作用。结果表明，单一化合物对厌氧氨氧化的抑制作用可以很好地符合扩展非竞争和 Luong 抑制动力学模型。各化合物的抑制作用顺序为：苯>甲苯>苯酚>苯甲酸。苯与甲苯、苯与苯酚和苯与苯甲酸的二元混合物对厌氧氨氧化活性的联合抑制作用呈加和性；三元混合物（苯、甲苯和苯酚）的联合抑制作用部分加和；而多组分混合物（苯、甲苯、苯酚和苯甲酸）的联合抑制作用则为协同作用。通过长期试验评估了苯甲酸对苯的反硝化-厌氧氨氧化协同降解的影响。尽管添加 10 mg/L 苯甲酸会使苯的降解平均速率降低 13%，但 NO 和 NH 的平均速率分别增加了约 1 倍和 0.56 倍，这表明苯甲酸有利于反硝化-厌氧氨氧化协同作用的稳定性。在反硝化协同厌氧氨氧化条件下，苯的羧化可能是苯厌氧降解的更有利途径。
