Trautwein Kathleen, Rabus Ralf
Institute for Chemistry and Biology of the Marine Environment (ICBM), Carl von Ossietzky University Oldenburg, Oldenburg, Germany.
Methods Mol Biol. 2018;1841:95-112. doi: 10.1007/978-1-4939-8695-8_8.
OMICs-based investigations of microorganisms are becoming more and more widespread in the upcoming era of systems and synthetic biology. Here, proteomics plays a key role and two-dimensional difference gel electrophoresis (2D DIGE) remains the "gold-standard" for globally determining protein abundance changes on a quantitative and statistically confident level-in particular also for laboratories not having full-cycle proteomic facilities at their disposal. In this contribution we summarize our methodological procedures and experiences with 2D DIGE accumulated over the past 15 years.
在即将到来的系统生物学和合成生物学时代，基于组学的微生物研究正变得越来越普遍。在这里，蛋白质组学起着关键作用，二维差异凝胶电泳（2D DIGE）仍然是在定量和统计可靠水平上全局确定蛋白质丰度变化的“金标准”——特别是对于那些没有全套蛋白质组学设施可供使用的实验室。在本论文中，我们总结了过去15年中积累的关于2D DIGE的方法步骤和经验。
