Muroi Makoto, Osada Hiroyuki
Chemical Biology Research Group, RIKEN CSRS, Wako, Saitama, Japan.
Methods Mol Biol. 2019;1888:127-139. doi: 10.1007/978-1-4939-8891-4_7.
Recent improvements in technologies such as omics analysis have enabled us to acquire a large amount of data regarding the biological changes in cells treated with bioactive small molecules. Using such data, a variety of profiling methods have been established for target identification of such bioactive compounds. In this chapter, we describe a proteomic profiling system, ChemProteoBase, based on proteome analysis using two-dimensional difference gel electrophoresis. This system compares the similarities in protein expression of 296 spots detected in the gel among the test compounds.
诸如组学分析等技术的最新进展使我们能够获取大量有关用生物活性小分子处理的细胞中生物学变化的数据。利用这些数据，已经建立了多种用于此类生物活性化合物靶点鉴定的分析方法。在本章中，我们描述了一种基于二维差异凝胶电泳蛋白质组分析的蛋白质组分析系统ChemProteoBase。该系统比较了测试化合物之间在凝胶中检测到的296个斑点的蛋白质表达相似性。
