微生物中的磷酸化蛋白质组学：研究天蓝色链霉菌分化的实验方案。
Phosphoproteomics in Microbiology: Protocols for Studying Streptomyces coelicolor Differentiation.
作者信息
Manteca Angel, Rioseras Beatriz, Rogowska-Wrzesinska Adelina, Jensen Ole N
机构信息
Area de Microbiologia, Departamento de Biologia Funcional, Facultad de Medicina, IUOPA, Universidad de Oviedo, Oviedo, Spain.
Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark.
出版信息
Methods Mol Biol. 2018;1841:249-260. doi: 10.1007/978-1-4939-8695-8_17.
The extension and biological role of Ser/Thr/Tyr phosphorylation in prokaryotes have been only scarcely studied. In this chapter, we describe the state of the art of microbial phosphoproteomics, focusing on protocols used for studying the phosphoproteome of Streptomyces coelicolor, one of the bacteria encoding the largest number of eukaryote-like Ser/Thr/Tyr kinases.
原核生物中丝氨酸/苏氨酸/酪氨酸磷酸化的扩展及其生物学作用仅得到了极少的研究。在本章中，我们描述了微生物磷酸化蛋白质组学的现状，重点关注用于研究天蓝色链霉菌磷酸化蛋白质组的实验方案，天蓝色链霉菌是编码数量最多的类真核生物丝氨酸/苏氨酸/酪氨酸激酶的细菌之一。
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