Keiblinger Katharina M, Riedel Katharina
Department of Forest and Soil Sciences, Institute of Soil Research, University of Natural Resources and Life Sciences Vienna (BOKU), Vienna, Austria.
Department of Microbial Physiology and Molecular Biology, Institute for Microbiology, University Greifswald, Greifswald, Germany.
Methods Mol Biol. 2018;1841:303-318. doi: 10.1007/978-1-4939-8695-8_21.
Soil and litter metaproteomics, assigning soil and litter proteins to specific phylogenetic and functional groups, has a great potential to shed light on the impact of microbial diversity on soil ecosystem functioning. However, metaproteomic analysis of soil and litter is often hampered by the enormous heterogeneity of the soil matrix and high concentrations of humic acids. To circumvent these challenges, sophisticated protocols for sample preparation have to be applied. This chapter provides the reader with detailed information on well-established protocols for protein extraction from soil and litter samples together with protocols for further sample preparation for subsequent MS analyses.
土壤和凋落物宏蛋白质组学可将土壤和凋落物中的蛋白质归属于特定的系统发育和功能组，在揭示微生物多样性对土壤生态系统功能的影响方面具有巨大潜力。然而，土壤和凋落物的宏蛋白质组分析常常受到土壤基质巨大的异质性和高浓度腐殖酸的阻碍。为克服这些挑战，必须应用复杂的样品制备方案。本章为读者提供了关于从土壤和凋落物样品中提取蛋白质的成熟方案的详细信息，以及用于后续质谱分析的进一步样品制备方案。
