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[应用琼脂扩散沉淀法检测轮状病毒抗原]

[Use of the agar diffusion precipitation method for detecting rotavirus antigen].

作者信息

Gracheva N M, Demidova S A, Blokhina T A, Avakov A A, Eliseeva I Ia

出版信息

Vopr Virusol. 1986 Sep-Oct;31(5):629-31.

PMID:3026098
Abstract

The possibility of rotavirus detection in feces of patients with acute enteric diseases by the agar gel diffusion (AGD) test was studied. The effectiveness of this method was compared with that of the standard method, direct electron microscopy. Both methods showed good correlation of the results, but the AGD test is methodically much simpler which recommends it for diagnosis of rotavirus infection. Rotavirus-specific hyperimmune calf serum may by used as a serological diagnostic preparation for the detection of human rotavirus antigen.

摘要

研究了通过琼脂凝胶扩散(AGD)试验检测急性肠道疾病患者粪便中轮状病毒的可能性。将该方法的有效性与标准方法直接电子显微镜检查进行了比较。两种方法的结果显示出良好的相关性,但AGD试验在方法上要简单得多,因此推荐用于诊断轮状病毒感染。轮状病毒特异性超免疫小牛血清可用作检测人轮状病毒抗原的血清学诊断制剂。

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