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[用于诊断人轮状病毒感染的免疫酶分析异源系统及其与RNA电泳的比较]

[Heterologous system of immunoenzyme analysis for the diagnosis of human rotavirus infection and its comparison with RNA electrophoresis].

作者信息

Bukrinskaia A G, Rukhadze G G, Selivanov Ia M, Molibog E V, Tentsov Iu Iu

出版信息

Vopr Virusol. 1988 Jan-Feb;33(1):52-8.

PMID:2835872
Abstract

On the basis of porcine rotavirus a heterologous EIA test system was worked out and tested for diagnosis of human rotavirus infection. A high sensitivity and specificity of the test system was demonstrated, its results were compared with those of electron microscopy, diffuse precipitation test, and RNA electrophoresis. Out of 201 specimens (fecal filtrates) collected from children ranging in ages from 14 days to 10 years, rotavirus antigen was detected in 68 (33.8%) RNA electrophoresis demonstrated the rotaviruses circulating in Moscow to have basically the same electrophoretic type, although rotaviruses with other electrophoretic types, including the "short" electrophoretic type, were also detected.

摘要

基于猪轮状病毒建立了一种异源酶免疫分析检测系统,并对其用于诊断人类轮状病毒感染进行了测试。该检测系统显示出高灵敏度和特异性,其结果与电子显微镜检查、琼脂扩散沉淀试验和RNA电泳的结果进行了比较。在从14天至10岁儿童收集的201份标本(粪便滤液)中,68份(33.8%)检测到轮状病毒抗原。RNA电泳表明,莫斯科流行的轮状病毒基本具有相同的电泳类型,不过也检测到了其他电泳类型的轮状病毒,包括“短”电泳类型。

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