Bellamy K, Gardner P S, Hambling M H, Rice S, Bradburne A F
J Virol Methods. 1983 Aug;7(2):65-72. doi: 10.1016/0166-0934(83)90093-9.
A simple method for the detection of human rotavirus in stools is described, using a double antibody sandwich enzyme-linked immunosorbent assay. Polysterene microtitre plates were used as solid phase. Four capture antibodies were tried, bovine, egg-derived, guinea pig and monoclonal antibody to rotavirus. Both bovine and egg-derived antirotavirus labelled with horseradish peroxidase were used as the detecting antibodies. The results obtained were compared with a commercially available ELISA, Rotazyme (Abbott Laboratories), and also with the direct detection of rotavirus by electron microscopy. Bovine antibody was found to be an unsuitable capture antibody due to non-specific false positive reactions.
本文描述了一种使用双抗体夹心酶联免疫吸附测定法检测粪便中人类轮状病毒的简单方法。使用聚苯乙烯微量滴定板作为固相。尝试了四种捕获抗体，即牛源、卵源、豚鼠源抗轮状病毒抗体以及抗轮状病毒单克隆抗体。用辣根过氧化物酶标记的牛源和卵源抗轮状病毒抗体均用作检测抗体。将所得结果与市售酶联免疫吸附测定试剂盒Rotazyme（雅培实验室）进行比较，同时也与通过电子显微镜直接检测轮状病毒的结果进行比较。发现牛源抗体由于非特异性假阳性反应而不适合作捕获抗体。
