莫洛尼氏鼠肉瘤病毒的温度依赖性杀细胞效应
Temperature-dependent cytocidal effects of Moloney murine sarcoma virus.
作者信息
Escobedo J, Singh B, Dina D, Arlinghaus R B
出版信息
Virus Res. 1986 Oct;6(1):75-84. doi: 10.1016/0168-1702(86)90058-4.
PMID:3026112
Abstract
Infection of L6E9 rat myoblasts with Moloney murine sarcoma virus (Mo-MuSV) at 37 degrees C resulted in the killing of target cells in less than one week. However, cells infected at 37 degrees C and shifted to 40 degrees C were not killed but underwent morphological transformation and had reduced levels of virus-specific RNA. A marked decrease in a cellular 55 kDa protein, as judged by immunoblotting, correlated with increased expression of Mo-MuSV RNA in infected cells.
摘要
在37摄氏度下用莫洛尼氏鼠肉瘤病毒（Mo-MuSV）感染L6E9大鼠成肌细胞，不到一周就导致靶细胞死亡。然而，在37摄氏度下感染并转移到40摄氏度的细胞并未死亡，而是发生了形态转化，且病毒特异性RNA水平降低。通过免疫印迹判断，一种细胞55 kDa蛋白显著减少，这与感染细胞中Mo-MuSV RNA表达增加相关。
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