二甲基亚砜对人肝癌细胞系Hep G2血红素合成的诱导作用：一个转录激活事件
Induction of haem synthesis in Hep G2 human hepatoma cells by dimethyl sulphoxide. A transcriptionally activated event.
作者信息
Galbraith R A, Sassa S, Kappas A
出版信息
Biochem J. 1986 Jul 15;237(2):597-600. doi: 10.1042/bj2370597.
Abstract
Exposure of cultured human hepatoma cells (Hep G2) to medium containing 2% (v/v) dimethyl sulphoxide resulted in an approximate doubling in the activity of delta-aminolaevulinate dehydratase, an increase in the haem content and a decreased growth rate; induced enzyme activity was decrease by 50% after treatment with alpha-amanitin. The findings are strikingly similar to those seen in murine Friend-virus-transformed erythroleukaemia cells.
摘要
将培养的人肝癌细胞（Hep G2）暴露于含有2%（v/v）二甲基亚砜的培养基中，导致δ-氨基乙酰丙酸脱水酶的活性增加约一倍，血红素含量增加，生长速率降低；用α-鹅膏蕈碱处理后，诱导的酶活性降低了50%。这些发现与在鼠类Friend病毒转化的红白血病细胞中观察到的结果惊人地相似。
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