Yang Xiaojuan, Wu Qingping, Zhang Jumei, Guo Weipeng, Mo Shuping, Liu Shengrong
Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou, 510070 China.
Food Sci Biotechnol. 2016 Aug 31;25(4):1109-1113. doi: 10.1007/s10068-016-0178-4. eCollection 2016.
This study aimed to classify a collection of () strains previously identified from powdered infant formula (PIF) to species level by gene sequencing and biochemical testing to determine the distribution of species in China and investigate the strain diversity by cellular fatty acid (CFA) analysis. Of 24 isolates, 23 were identified as and one was . The 23 isolates showed the same CFA profiles. The isolate was discriminated from the isolates by the significant difference in the amounts of C, C, and C acids. These results showed that and were the common Cronobacter species distributed in PIF in China and that the isolates of the two species exhibited different CFA profiles. These findings are of value for epidemiological investigations and provide an alternative method for confirming various spp.
本研究旨在通过基因测序和生化测试将先前从婴儿配方奶粉(PIF)中鉴定出的一组()菌株分类到种水平,以确定在中国的分布情况,并通过细胞脂肪酸(CFA)分析研究菌株多样性。在24株分离株中,23株被鉴定为,1株为。23株分离株显示出相同的CFA谱。该分离株通过C、C和C酸含量的显著差异与分离株区分开来。这些结果表明,和是在中国PIF中分布的常见阪崎肠杆菌物种,并且这两个物种的分离株表现出不同的CFA谱。这些发现对流行病学调查具有价值,并为确认各种阪崎肠杆菌属物种提供了一种替代方法。