Iversen Carol, Mullane Niall, McCardell Barbara, Tall Ben D, Lehner Angelika, Fanning Séamus, Stephan Roger, Joosten Han
Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, CH-8057, Zurich, Switzerland.
Int J Syst Evol Microbiol. 2008 Jun;58(Pt 6):1442-7. doi: 10.1099/ijs.0.65577-0.
[Enterobacter] sakazakii is an opportunistic pathogen that can cause infections in neonates. This study further clarifies the taxonomy of isolates described as [E.] sakazakii and completes the formal description of the proposed reclassification of these organisms as novel species and subspecies within a proposed novel genus, Cronobacter gen. nov. [E.] sakazakii was first defined in 1980, however recent polyphasic taxonomic analysis has determined that this group of organisms consists of several genomospecies. In this study, the phenotypic descriptions of the proposed novel species are expanded using Biotype 100 and Biolog Phenotype MicroArray data. Further DNA-DNA hybridization experiments showed that malonate-positive strains within the [E.] sakazakii genomospecies represent a distinct species, not a subspecies. DNA-DNA hybridizations also determined that phenotypically different strains within the proposed species, Cronobacter dublinensis sp. nov., belong to the same species and can be considered as novel subspecies. Based on these analyses, the following alternative classifications are proposed: Cronobacter sakazakii gen. nov., comb. nov. [type strain ATCC 29544(T) (=NCTC 11467(T))]; Cronobacter malonaticus sp. nov. [type strain CDC 1058-77(T) (=LMG 23826(T)=DSM 18702(T))]; Cronobacter turicensis sp. nov. [type strain z3032(T) (=LMG 23827(T)=DSM 18703(T))]; Cronobacter muytjensii sp. nov. [type strain ATCC 51329(T) (=CIP 103581(T))]; Cronobacter dublinensis sp. nov. [type strain DES187(T) (=LMG 23823(T)=DSM 18705(T))]; Cronobacter dublinensis subsp. dublinensis subsp. nov. [type strain DES187(T) (=LMG 23823(T)=DSM 18705(T))]; Cronobacter dublinensis subsp. lausannensis subsp. nov. [type strain E515(T) (=LMG 23824=DSM 18706(T))], and Cronobacter dublinensis subsp. lactaridi subsp. nov. [type strain E464(T) (=LMG 23825(T)=DSM 18707(T))].
阪崎肠杆菌是一种可导致新生儿感染的机会致病菌。本研究进一步阐明了被描述为阪崎肠杆菌的菌株的分类，并完成了对这些微生物重新分类为一个新提出的属——克罗诺杆菌属（Cronobacter gen. nov.）内的新物种和亚种的正式描述。阪崎肠杆菌于1980年首次被定义，然而最近的多相分类分析已确定这一组微生物由几个基因种组成。在本研究中，利用100型生物分型和Biolog表型微阵列数据扩展了对新提出物种的表型描述。进一步的DNA - DNA杂交实验表明，阪崎肠杆菌基因种内的丙二酸盐阳性菌株代表一个独特的物种，而非亚种。DNA - DNA杂交还确定了新提出的都柏林克罗诺杆菌（Cronobacter dublinensis sp. nov.）物种内表型不同的菌株属于同一物种，可被视为新亚种。基于这些分析，提出了以下分类变更：阪崎克罗诺杆菌（Cronobacter sakazakii gen. nov., comb. nov.）[模式菌株ATCC 29544(T) (=NCTC 11467(T))]；丙二酸克罗诺杆菌（Cronobacter malonaticus sp. nov.）[模式菌株CDC 1058 - 77(T) (=LMG 23826(T)=DSM 18702(T))]；苏黎世克罗诺杆菌（Cronobacter turicensis sp. nov.）[模式菌株z3032(T) (=LMG 23827(T)=DSM 18703(T))]；穆氏克罗诺杆菌（Cronobacter muytjensii sp. nov.）[模式菌株ATCC 51329(T) (=CIP 103581(T))]；都柏林克罗诺杆菌（Cronobacter dublinensis sp. nov.）[模式菌株DES187(T) (=LMG 23823(T)=DSM 18705(T))]；都柏林克罗诺杆菌都柏林亚种（Cronobacter dublinensis subsp. dublinensis subsp. nov.）[模式菌株DES187(T) (=LMG 23823(T)=DSM 18705(T))]；都柏林克罗诺杆菌洛桑亚种（Cronobacter dublinensis subsp. lausannensis subsp. nov.）[模式菌株E515(T) (=LMG 23824=DSM 18706(T))]，以及都柏林克罗诺杆菌乳酸亚种（Cronobacter dublinensis subsp. lactaridi subsp. nov.）[模式菌株E464(T) (=LMG 23825(T)=DSM 18707(T))]。
