一种高产莫纳可林K但不产桔霉素的MS-1菌株。
A MS-1 strain with high-yield monacolin K but no citrinin.
作者信息
Feng Yanli, Chen Wanping, Chen Fusheng
机构信息
1Key Laboratory of Environment Correlative Dietology, Ministry of Education, Huazhong Agricultural University, Wuhan, 430070 China.
2Hubei Key Laboratory of Edible Wild Plants Conservation & Utilization (Hubei Normal University), Huangshi, 435002 China.
出版信息
Food Sci Biotechnol. 2016 Aug 31;25(4):1115-1122. doi: 10.1007/s10068-016-0179-3. eCollection 2016.
Abstract
Monacolin K (MK) produced by spp. is mostly used to regulate cholesterol level, while it can be contaminated by citrinin, a mycotoxin yielded by some strains. To develop production of citrinin-free MK, an . sp. MS-1 strain, identified as by morphologies and internal transcribed spacer sequences analysis, was isolated from red fermented rice. After 14 days of fermentation, yields of citrinin-free MK could be up to 0.58 mg/mL and 16.45 mg/g in liquid- and solidstate fermentation products under optimal conditions, respectively. Further determination revealed that no citrinin biosynthesis related genes such as , , , and were detected. Thus, HPLC combined with citrinin-related gene analyses can be used for rapid screening of non-citrinin production strains.
摘要
由 种产生的莫纳可林K（MK）主要用于调节胆固醇水平，而它可能会被桔青霉素污染，桔青霉素是一些 菌株产生的一种霉菌毒素。为了开发无桔青霉素的MK生产工艺，从红曲米中分离出一株 . sp. MS-1菌株，通过形态学和内转录间隔区序列分析鉴定为 。在最佳条件下发酵14天后，无桔青霉素的MK在液体和固体发酵产物中的产量分别可达0.58 mg/mL和16.45 mg/g。进一步检测发现，未检测到与桔青霉素生物合成相关的基因，如 、 、 和 。因此，高效液相色谱法结合桔青霉素相关基因分析可用于快速筛选不产生桔青霉素的 菌株。
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