Yang Jian, Chen Qi, Wang Weiping, Hu Jiajun, Hu Chuan
Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei University of Technology, Wuhan 430068, China.
Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei University of Technology, Wuhan 430068, China.
J Biosci Bioeng. 2015 May;119(5):564-9. doi: 10.1016/j.jbiosc.2014.10.014. Epub 2014 Dec 5.
The influence of oxygen supply on Monascus pigments and citrinin production by Monascus ruber HS.4000 in submerged fermentation was studied. For Monascus cultivation with high pigments and low citrinin production, the initial growth phase, mid-stage phase, and later-stage production phase were separated by shifting oxygen supply. The optimal condition for the fermentation process in shake-flask fermentation was a three-stage rotating rate controlled strategy (0-48 h at 150 rpm, 48-108 h at 250 rpm, 108-120 h at 200 rpm) with medium volume of 100 mL added to 250 mL Erlenmeyer flasks at 30°C for 120 h cultivation. Compared to constant one-stage cultivation (medium volume of 100 mL, rotating rate of 250 rpm), the pigments were reduced by 40.4%, but citrinin was reduced by 64.2%. The most appropriate condition for the fermentation process in a 10 L fermentor is also a three-stage aeration process (0-48 h at 300 L/h, 48-96 h at 500 L/h, 96-120 h at 200 L/h) with agitation of 300 rpm at 30°C for 120 h cultivation, and 237.3 ± 5.7 U/mL pigments were produced in 120 h with 6.05 ± 0.19 mg/L citrinin in a 10 L fermentor. Compared to aeration-constant (500 L/h) cultivation, pigment production was increased by 29.6% and citrinin concentration was reduced by 79.5%.
研究了氧气供应对红曲霉菌HS.4000在深层发酵中产生红曲色素和桔霉素的影响。为实现红曲霉菌的高色素产量和低桔霉素产量，通过改变氧气供应来区分初始生长阶段、中期阶段和后期生产阶段。摇瓶发酵中发酵过程的最佳条件是采用三阶段转速控制策略（0 - 48小时为150转/分钟，48 - 108小时为250转/分钟，108 - 120小时为200转/分钟），将100 mL培养基加入到250 mL三角瓶中，在30°C下培养120小时。与恒定的单阶段培养（培养基体积100 mL，转速250转/分钟）相比，色素产量降低了40.4%，但桔霉素产量降低了64.2%。10 L发酵罐中发酵过程的最合适条件也是三阶段通气过程（0 - 48小时为300 L/小时，48 - 96小时为500 L/小时，96 - 120小时为200 L/小时），在30°C下以300转/分钟搅拌培养120小时，10 L发酵罐在120小时内产生237.3 ± 5.7 U/mL的色素，桔霉素含量为6.05 ± 0.19 mg/L。与恒定通气（500 L/小时）培养相比，色素产量提高了29.6%，桔霉素浓度降低了79.5%。
