Chi Yu-Yang, Shen Jing-Lin, Zhang Jing, Shan An-Shan, Niu Shu-Ling, Zhou Chang-Hai, Lee Hong-Gu, Jin Yong-Cheng
1Department of Animal Science, College of Animal Sciences, Jilin University, Changchun, 130062 China.
2Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030 China.
Food Sci Biotechnol. 2016 Aug 31;25(4):1147-1153. doi: 10.1007/s10068-016-0183-7. eCollection 2016.
The aim of this study is to reveal the relation among villin 2, Wnt/β-catenin, and adipogenesis by adding appropriate lithium chloride (LiCl). The study comprises three parts: the selection of LiCl concentration, the effect of LiCl on adipocyte differentiation during and after differentiation induction. By comprehensively analyzing the results of the experiments, we proved that LiCl can inhibit adipocyte differentiation and enhance villin 2 and β-catenin expressions not only during differentiation induction but also after it. Moreover, villin 2 has a significant impact on β-catenin. We suggest that villin 2 may participate in Wnt/β-catenin signaling.
本研究的目的是通过添加适当的氯化锂（LiCl）来揭示绒毛蛋白2、Wnt/β-连环蛋白与脂肪生成之间的关系。该研究包括三个部分：LiCl浓度的选择、LiCl在分化诱导期间及之后对脂肪细胞分化的影响。通过综合分析实验结果，我们证明LiCl不仅在分化诱导期间而且在诱导之后都能抑制脂肪细胞分化并增强绒毛蛋白2和β-连环蛋白的表达。此外，绒毛蛋白2对β-连环蛋白有显著影响。我们认为绒毛蛋白2可能参与Wnt/β-连环蛋白信号传导。
