Department of Animal Science/Bio-resources and Development Institute Pusan National University, Miryang, Gyeongnam 627-706, Korea.
BMB Rep. 2012 Feb;45(2):102-7. doi: 10.5483/BMBRep.2012.45.2.102.
The aim of this study was to investigate protein profiles related to the induction of adipogenesis within the bovine longissimus dorsi muscle (BLDM) by proteomic analysis. We analyzed BLDM proteins at different growth stages to clarify the physiological mechanisms of marbled muscle development in 20 head of Korean native cattle (11 month: 10 head, 17 month: 10 head). BLDM proteins were analyzed by two-dimensional electrophoresis and image analysis. Villin 2 was specifically identified by mass spectrometry and a protein search engine. Villin 2 protein expression in BLDM decreased during the fat development stage in test steers. In a Western blot cell culture study of spontaneously immortal bovine muscle fibroblasts, the abundance of Villin 2 was shown to be down-regulated during differentiation into muscle. In 3T3-L1 mouse embryonic fibroblasts, Villin 2 was decreased during differentiation into adipocytes. The results suggest that Villin 2 may be related to the induction of transdifferentiation and adipogenesis in bovine longissimus dorsi muscle.
本研究旨在通过蛋白质组学分析研究与牛背最长肌（BLDM）中脂肪生成诱导相关的蛋白质谱。我们分析了不同生长阶段的 BLDM 蛋白质，以阐明韩国本土牛（11 月龄：10 头，17 月龄：10 头）大理石状肌肉发育的生理机制。通过二维电泳和图像分析分析 BLDM 蛋白质。通过质谱和蛋白质搜索引擎特异性鉴定出肌动蛋白 2。在试验牛的脂肪发育阶段，BLDM 中的肌动蛋白 2 蛋白表达减少。在自发永生化牛肌肉成纤维细胞的 Western blot 细胞培养研究中，肌动蛋白 2 的丰度在分化为肌肉时下调。在 3T3-L1 小鼠胚胎成纤维细胞中，肌动蛋白 2 在分化为脂肪细胞时减少。结果表明，肌动蛋白 2 可能与牛背最长肌的诱导性转分化和脂肪生成有关。
