大鼠胸腺细胞的放射敏感性。
The radiosensitivity of rat thymocytes.
作者信息
Ord M G, Stocken L A
出版信息
Biochem J. 1986 Sep 1;238(2):517-21. doi: 10.1042/bj2380517.
gamma-Irradiation in vitro apparently blocked a plasma-membrane associated, superoxide-producing, NADPH oxidase in rat thymocytes. Differential centrifugation of the mixed thymocytes indicated the smaller lymphocytes (approx. 6 microns diameter) to be the radiosensitive population. The oxidase system co-isolated in part with thymus nuclei and could be solubilized by detergent treatment [Bellavite, Jones, Cross, Papini & Rossi (1984) Biochem. J. 223, 639-648]. Endogenous NADPH was the rate-limiting component for superoxide formation in vitro. The level of NADPH was lowered by gamma-irradiation, an effect mimicked by GSSG in the presence of 50 microM-ZnCl2 to inhibit GSSG reductase. These findings are suggested as the metabolic basis for interphase death of small lymphocytes exposed to ionizing radiation.
体外γ射线照射明显阻断了大鼠胸腺细胞中一种与质膜相关的、产生超氧化物的NADPH氧化酶。对混合胸腺细胞进行差速离心表明，较小的淋巴细胞（直径约6微米）是放射敏感群体。该氧化酶系统部分与胸腺核共同分离，并且可以通过去污剂处理使其溶解[贝拉维特、琼斯、克罗斯、帕皮尼和罗西（1984年）《生物化学杂志》223卷，639 - 648页]。内源性NADPH是体外超氧化物形成的限速成分。γ射线照射会降低NADPH的水平，在存在50微摩尔/升氯化锌以抑制谷胱甘肽还原酶的情况下，谷胱甘肽二硫化物（GSSG）也会模拟这种效应。这些发现被认为是暴露于电离辐射的小淋巴细胞间期死亡的代谢基础。
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